foci analysis

Previous Topic Next Topic
 
classic Classic list List threaded Threaded
1 message Options
Reply | Threaded
Open this post in threaded view
|

foci analysis

sequentialrant
Hello,

I am new to the ImageJ environment.

I am doing the quantitative evaluation of the DNA double strand breaks using immunohistochemistry.

I have a sequence of fluoresced image slices of nucleus acquired, each focussed at different depth, forming a stack of images.

I would appreciate if somebody could advice or give me a tip on how to accomplish the following tasks:

1. Assembling the in-focus portions of the sliced images to form a single image.

2. Applying a method or set of methods on the assembled image that automatically counts the number of foci, giving their intensity, and spatial distribution.

Thank you,
Nidhi