|
Hello,
I am new to the ImageJ environment.
I am doing the quantitative evaluation of the DNA double strand breaks using immunohistochemistry.
I have a sequence of fluoresced image slices of nucleus acquired, each focussed at different depth, forming a stack of images.
I would appreciate if somebody could advice or give me a tip on how to accomplish the following tasks:
1. Assembling the in-focus portions of the sliced images to form a single image.
2. Applying a method or set of methods on the assembled image that automatically counts the number of foci, giving their intensity, and spatial distribution.
Thank you,
Nidhi
|