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Dear Image J users and developers,
I really need your help. I have been looking around in the available
plugins and I couldn't find a way of doing the following:
I have an RGB stack with 3 channels. Each channel is a binary file, so
the whole set of stacks only has 8 colour: red, green, blue, yellow
(colocalization of red+green), white (blue+red+green), magenta (red
+blue),light blue (green+blue) and black.
I need to know how many pixels of each color i have in the total stack
so that I can calculate the volumes in µm^3 of each of the colors. I
found that with the colocalization analisys I can do this for stacks
with only 4 colours (red, green, black, yellow) ,but it only admits 2
channel not three and therefore, i coulnd't find a way of doing it
with three colors at the same time and it is especially important for
me to know the volume of the white pixels. (I know that otherwise I
could just calculate separately first red+blue, and I would get: red,
blue and magenta; and red+green, to get red, green and yellow; and
green+blue, to get light blue) but I could get a way of knowing which
one is the volume of white pixels.
As well I thought that I might be able to do it with the tool Object
counter 3D, but I don't know why it doesn't work for me, whenever I
try to use it, the following error pops out:
java.lang.ClassFormatError: Incompatible magic value -1095041334 in
class file Object_Counter3D
at java.lang.ClassLoader.defineClass1(Native Method)
at java.lang.ClassLoader.defineClass(ClassLoader.java:675)
at java.lang.ClassLoader.defineClass(ClassLoader.java:520)
at ij.io.PluginClassLoader.loadClass(PluginClassLoader.java:246)
at ij.io.PluginClassLoader.loadClass(PluginClassLoader.java:209)
at ij.IJ.runUserPlugIn(IJ.java:156)
at ij.IJ.runPlugIn(IJ.java:124)
at ij.Executer.runCommand(Executer.java:104)
at ij.Executer.run(Executer.java:58)
at java.lang.Thread.run(Thread.java:613)
Thank you very much for your help. I really need to do this
computation and Image J is the only program I can think of able of
doing this.
Best,
Lucia
Lucia Prieto Godino, PhD student
Department of Zoology, University of Cambridge
Downing street CB23EJ Cambridge, UK
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