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help interpreting particle count output

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help interpreting particle count output

Mary Heng
1 post
Dear users,

I am new to Image and need help interpreting the Particle Counting output. I have set my scale to 100um, threshold my image and used the analyze particle function. The output gives for example, a total area of 92,758 is this in pixels or in um or per 100um? see below.

Slice Count Total Area Average Size Area Fraction Mean
        198          92,758            468.475     8.6         255

I appreciate any clarification.

Thank you for your help,

Mary
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Re: help interpreting particle count output

BenTupper
122 posts
Hi,

On May 23, 2010, at 1:04 AM, Mary Heng wrote:

> Dear users,
>
> I am new to Image and need help interpreting the Particle Counting  
> output. I have set my scale to 100um, threshold my image and used  
> the analyze particle function. The output gives for example, a total  
> area of 92,758 is this in pixels or in um or per 100um? see below.
>
> Slice Count Total Area Average Size Area Fraction Mean
> 198          92,758            468.475     8.6         255
>
> I appreciate any clarification.
>

Hi,

The online documentation...

http://rsb.info.nih.gov/ij/docs/menus/analyze.html#set

and the examples in ...

http://rsb.info.nih.gov/ij/docs/pdfs/examples.pdf

will help clarify this for you.

CHeers,
Ben