Curious if anyone else has seen this problem.
We have about 200 CZI files from Zeiss Lightsheet that are 2 or 4 channels and 100 to 300 Z slices. Each one 10 to 30 GB.
We have a macro that opens each one, crops it, sometimes scales it, sets display mode composite, assigns a color and contrast range to each channel, sometimes converted to RGB, and saves with a new file name (i.e. original + "processed"). After each file is processed, a call is made to garbage collect before the next file is opened.
Some of the images end up with horizontal saturated lines at apparently random locations, not always the full width of the image.
We are using a version of IJ 1.50 posted within the last month on Win 7 with the version of Java bundled with IJ.
I'm thinking of trying a work around splitting the channels, processing, then reassembling, to see if this helps, but first wanted to see if anyone else has encountered this.
Thanks!
_________________________________________
Michael Cammer, Optical Microscopy Specialist
http://ocs.med.nyu.edu/microscopyhttp://microscopynotes.com/Cell: (914) 309-3270
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