how imageJ process 16 bit gray scale image?

[ashishdonvir]

When i load my 16 bit gray scale image into imageJ it actually looks bit darker then original image and what ever i process on to image like Unsharp mask,brightness,contrast it performes in this darker loaded image. but when i save it as .tiff this image it actually does all this process on my actual image wich is quite brighter then the loaded image.  
so why does imajeJ behave like this?why it is not processing my actual 16 bit gray scale image?

i will be glade if some body answer me.  

 

[Michael Schmid]

Hi Ashish (did I guess your name correctly?)

by default, ImageJ opens 16-bit images with black corresponding to 0 and whit to 65535.
You can change the displayed range by Image>Adjust>Brightness/Contrast; for 16-bit images this will modify only the display, not the image data.

You may also have a look at this post to the mailinglist:
  http://imagej.1557.n6.nabble.com/TIFF-12bit-16bit-display-conversion-problems-td3696612.html

Michael
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On Apr 20, 2012, at 08:40, ashishdonvir wrote:

> When i load my 16 bit gray scale image into imageJ it actually looks bit
> darker then original image and what ever i process on to image like Unsharp
> mask,brightness,contrast it performes in this darker loaded image. but when
> i save it as .tiff this image it actually does all this process on my actual
> image wich is quite brighter then the loaded image.  
> so why does imajeJ behave like this?why it is not processing my actual 16
> bit gray scale image?
>
> i will be glade if some body answer me.