Hi Vanessa,
as the name implies, RGB images have only 3 color channels, R, G and B.
Thus, if you convert an image to RGB, you will never be able to recover 4 independent channels from the RGB.
So you will have to reformulate your question:
Is there any stitching plugin that works with composite images having 4 channels?
It seems that some stitching plugins of Fiji can do this (but I have no experience with them, and anyhow I don't know what exactly you need)
http://fiji.sc/Image_StitchingMichael
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On Jun 14, 2015, at 22:51, Vanessa Punal wrote:
> Hi ImageJ Community,
>
>
> I am hoping someone can help me out with an issue I have encountered. I have taken several z-stack images which contains 4 channels (blue, green, red, and far red) and have maximum projected the data. Because I am stitching these images together, I need to convert each 4 channel maximum projection image into an RGB image. However, when I do this and then revert the image back to a composite, I always loose the 4th channel (in reality, the 4th channel becomes merged with the other 3 channels). How can I fix this? Thank you in advance!
>
>
> Best,
>
> Vanessa
>
>
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