how to measure the volume of the tissue

[Yisong Zhen-2]

Dear ImageJ users,

I want to measure the volume of the each mouse heart. I want to use digital
camera to take the photo of the tissue and extract the essential
information to calculate the volume.  How can I do and use which kind of
plugin to complete my task? Thanks.

--
//---------------------------------------------------------------------------------------
//   We have a hunger of the mind which asks for knowledge
//   of all around us, and the more we gain, the more is
//   our desire; the more we see, the more we are capable
//   of seeing.
//---------------------------------------------------------------------------------------
@
YiSong Zhen, Ph.D
Sino-German Laboratory for Molecular Medicine
Fu Wai Hospital
Beilishilu 167, 100037 Beijing
P.R.China
http://www.cardiosignal.org
Tel: [phone]
@----------------------------------------------------------------------------------------

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[Romain Guiet]

Hi YiSong,
you may have a look to stereology technics and the cavalieri estimator :
http://www.stereology.info/cavalieri-estimator/.
or you can try to measure the volume displacement to calculate the volume, using Archimedes Principle.
Cheers,
Romain


---------------------------------------------------------------
Dr. Romain Guiet
Bioimaging and Optics Platform (PT-BIOP)
Ecole Polytechnique Fédérale de Lausanne (EPFL)
Faculty of Life Sciences
Station 19, AI 0140
CH-1015 Lausanne

Phone: [+4121 69] 39629
http://biop.epfl.ch/
---------------------------------------------------------------

________________________________________
De : ImageJ Interest Group [[hidden email]] de la part de Yisong Zhen [[hidden email]]
Envoyé : mercredi 30 septembre 2015 07:45
À : [hidden email]
Objet : how to measure the volume of the tissue

Dear ImageJ users,

I want to measure the volume of the each mouse heart. I want to use digital
camera to take the photo of the tissue and extract the essential
information to calculate the volume.  How can I do and use which kind of
plugin to complete my task? Thanks.

--
//---------------------------------------------------------------------------------------
//   We have a hunger of the mind which asks for knowledge
//   of all around us, and the more we gain, the more is
//   our desire; the more we see, the more we are capable
//   of seeing.
//---------------------------------------------------------------------------------------
@
YiSong Zhen, Ph.D
Sino-German Laboratory for Molecular Medicine
Fu Wai Hospital
Beilishilu 167, 100037 Beijing
P.R.China
http://www.cardiosignal.org
Tel: [phone]
@----------------------------------------------------------------------------------------

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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[Yisong Zhen]

Dear Romain,

The mouse heart is so small and wet (with fixation buffer), and I am afraid
that system error would not allow me to accurately get the
volume information by Archimedes Principle. At least I have no
precision-proof equipment to measure the water volume. This might be a way
but it take time anyway. As your first proposal, if I understand correctly,
it need sample sliced. And this is not permitted. My question is: I have
digital camera and tiny mouse heart,  I want to take photos in several
positions of the heart under microscopy. From those digital information, I
want to get the volume of the mouse heart. Is there some plugin can do it?
Anyway, thanks again your suggestions.

Yisong

On Wed, Sep 30, 2015 at 3:16 PM, Romain Guiet <[hidden email]> wrote:

> Hi YiSong,
> you may have a look to stereology technics and the cavalieri estimator :
> http://www.stereology.info/cavalieri-estimator/.
> or you can try to measure the volume displacement to calculate the volume,
> using Archimedes Principle.
> Cheers,
> Romain
>
>
> ---------------------------------------------------------------
> Dr. Romain Guiet
> Bioimaging and Optics Platform (PT-BIOP)
> Ecole Polytechnique Fédérale de Lausanne (EPFL)
> Faculty of Life Sciences
> Station 19, AI 0140
> CH-1015 Lausanne
>
> Phone: [+4121 69] 39629
> http://biop.epfl.ch/
> ---------------------------------------------------------------
>
> ________________________________________
> De : ImageJ Interest Group [[hidden email]] de la part de Yisong
> Zhen [[hidden email]]
> Envoyé : mercredi 30 septembre 2015 07:45
> À : [hidden email]
> Objet : how to measure the volume of the tissue
>
> Dear ImageJ users,
>
> I want to measure the volume of the each mouse heart. I want to use digital
> camera to take the photo of the tissue and extract the essential
> information to calculate the volume.  How can I do and use which kind of
> plugin to complete my task? Thanks.
>
> --
>
> //---------------------------------------------------------------------------------------
> //   We have a hunger of the mind which asks for knowledge
> //   of all around us, and the more we gain, the more is
> //   our desire; the more we see, the more we are capable
> //   of seeing.
>
> //---------------------------------------------------------------------------------------
> @
> YiSong Zhen, Ph.D
> Sino-German Laboratory for Molecular Medicine
> Fu Wai Hospital
> Beilishilu 167, 100037 Beijing
> P.R.China
> http://www.cardiosignal.org
> Tel: [phone]
>
> @----------------------------------------------------------------------------------------
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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