image registration

[nicolanorthrop]

Hi

I have a sequence of images from an angiogram in a stack. I have used the process-image calculator in imagej to perform digital subtraction of the image without contrast in from the other images to leave just the contrast in the resulting image. There seems to be movement throughout the sequence which causes misregistration artefacts.

For the task i have been set i think i need to place markers on each image and then perform the digital subtraction as before, is there a way to do this?  i am a beginner to imagej and the only thing i have done previously with image registration is using TurboReg to just register two images.

Can anyone help please?

Thanks

Nicola

[Joel Sheffield]

There is a plugin related to TurboReg called Stackreg.  That might help you.
Joel


On Fri, Apr 29, 2011 at 9:32 AM, nicolanorthrop <[hidden email]
> wrote:

> Hi
>
> I have a sequence of images from an angiogram in a stack. I have used the
> process-image calculator in imagej to perform digital subtraction of the
> image without contrast in from the other images to leave just the contrast
> in the resulting image. There seems to be movement throughout the sequence
> which causes misregistration artefacts.
>
> For the task i have been set i think i need to place markers on each image
> and then perform the digital subtraction as before, is there a way to do
> this?  i am a beginner to imagej and the only thing i have done previously
> with image registration is using TurboReg to just register two images.
>
> Can anyone help please?
>
> Thanks
>
> Nicola--
> View this message in context:
> http://imagej.588099.n2.nabble.com/image-registration-tp6316910p6316910.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>

--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs

[nicolanorthrop]

Thanks, ill have a look at that and see how i get on.

Nicola

[Ignacio Arganda-Carreras]

You can check all the registration plugins available in Fiji:

http://pacific.mpi-cbg.de/wiki/index.php/Category:Registration

<http://pacific.mpi-cbg.de/wiki/index.php/Category:Registration>ignacio

On Fri, Apr 29, 2011 at 9:59 AM, JOEL B. SHEFFIELD <[hidden email]> wrote:

> There is a plugin related to TurboReg called Stackreg.  That might help
> you.
> Joel
>
>
> On Fri, Apr 29, 2011 at 9:32 AM, nicolanorthrop <
> [hidden email]
> > wrote:
>
> > Hi
> >
> > I have a sequence of images from an angiogram in a stack. I have used the
> > process-image calculator in imagej to perform digital subtraction of the
> > image without contrast in from the other images to leave just the
> contrast
> > in the resulting image. There seems to be movement throughout the
> sequence
> > which causes misregistration artefacts.
> >
> > For the task i have been set i think i need to place markers on each
> image
> > and then perform the digital subtraction as before, is there a way to do
> > this?  i am a beginner to imagej and the only thing i have done
> previously
> > with image registration is using TurboReg to just register two images.
> >
> > Can anyone help please?
> >
> > Thanks
> >
> > Nicola--
> > View this message in context:
> >
> http://imagej.588099.n2.nabble.com/image-registration-tp6316910p6316910.html
> > Sent from the ImageJ mailing list archive at Nabble.com.
> >
>
>
>
> --
>
>
> Joel B. Sheffield, Ph.D
> Department of Biology
> Temple University
> Philadelphia, PA 19122
> Voice: 215 204 8839
> e-mail: [hidden email]
> URL:  http://astro.temple.edu/~jbs
>

--
Ignacio Arganda-Carreras, Ph.D.
Seung's lab, 46-5065
Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology
43 Vassar St.
Cambridge, MA 02139
USA

Phone: [phone]
Website: http://bioweb.cnb.csic.es/~iarganda/index_EN.html