intensity correction of z stacks (linear, nonlinear)

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intensity correction of z stacks (linear, nonlinear)

Guenter Giese
Hi all,

It is common in confocal microscopy that, despite of careful selection of
embedding media, objectives etc., signal intensity drops with increasing
focus depth (e.g in thick tissue samples).

Is there any built-in function or a plugin available for intensity
correction of z stacks (linear, nonlinear)?

linear: scaling of intensities using a linear gradient
nonlinear: scaling, e.g. with a gamma function

Would such a function include conversion from integer to float and back to
integer intensity values?

Guenter


------------------------------------------
Dr. Guenter Giese
Light Microscopy Facility Manager
Dept. of Biomedical Optics
MPI fuer Medizinische Forschung Jahnstr. 29
D-69120 Heidelberg, Germany
Phone (+49) 6221-486-360 (Fax: -325)
e-mail: [hidden email]
http://lightmicro.mpimf-heidelberg.mpg.de