Hi Mario,
what about the following:
- Edit>Selection>Create selection to convert the mask into a selection
- Select the image of the proteins
- Edit>Selection>Restore Selection to transfer the selection
- Analyze>Histogram
Michael
________________________________________________________________
On 27 Nov 2007, at 22:13, mn79 (
[hidden email]) wrote:
> Is there a way to easily get a profile of pixel intensity
> distributions under
> a mask?
>
> I am working with a protein that shows different types of
> localization in
> different mutants. I would like to quantify the 'degree of
> clustering'. My
> thought was to quantify this by how much the pixel in each masked
> image
> deviates from the mean intensity.
>
> For example, a perfectly evenly distributed protein should show a
> sharp peak
> right around the mean intensity. An extremely unevenly distributed
> one (a
> single bright cluster) should show a bi-modal distribution with the
> majority
> of pixels having a lower intensity than the mean, while a small
> fraction of
> the pixels should be much brighter than the mean.
>
> So, is there a way to get such a pixel intensity histogram? Thank
> you for
> your help.
Locked
