islet area on H&E slide

[Creas]

Dear Sir,

I am a PhD student and I am trying to determine the size of each individual islet on a pancreas slide stained with H&E.  I wonder how should I do this as both the acinar and islets are stained with hematoxylin and eosin, and the adjacent blood vessels are also stained the same color as islets.

Please find below example of my slides.

Thank you.

Creas

[Wolfgang Schechinger]

Hi,

do you have the possibility of staining for insulin and/or glucagon instead?

For examples and details, see

http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=ueberberg-s

Best regards,

Wo

-------- Original-Nachricht --------
> Datum: Tue, 19 Jan 2010 00:27:27 -0800
> Von: Creas <[hidden email]>
> An: [hidden email]
> Betreff: islet area on H&E slide

> Dear Sir,
>
> I am a PhD student and I am trying to determine the size of each
> individual
> islet on a pancreas slide stained with H&E.  I wonder how should I do this
> as both the acinar and islets are stained with hematoxylin and eosin, and
> the adjacent blood vessels are also stained the same color as islets.
>
> Please find below example of my slides.
>
> Thank you.
>
> Creas http://n2.nabble.com/file/n4418723/624-5.jpg 
> --
> View this message in context:
> http://n2.nabble.com/islet-area-on-H-E-slide-tp4418723p4418723.html
> Sent from the ImageJ mailing list archive at Nabble.com.

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[Creas]

Dear Professor Schechinger,

Thank you very much for your advice.
Unfortunately I have already cut all my tissues and do not have any spare slides for insulin/glucagon counter stain.  I have another set of slides stained with insulin only.

Thank you.

Regards,
Creas




--- On Tue, 19/1/10, Wolfgang Schechinger [via ImageJ] <[hidden email]> wrote:

From: Wolfgang Schechinger [via ImageJ] <[hidden email]>
Subject: Re: islet area on H&E slide
To: "Creas" <[hidden email]>
Received: Tuesday, 19 January, 2010, 3:47 AM

Hi,

do you have the possibility of staining for insulin and/or glucagon instead?

For examples and details, see

http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=ueberberg-s

Best regards,

Wo

-------- Original-Nachricht --------
> Datum: Tue, 19 Jan 2010 00:27:27 -0800
> Von: Creas <[hidden email]>
> An: [hidden email]
> Betreff: islet area on H&E slide

> Dear Sir,
>
> I am a PhD student and I am trying to determine the size of each
> individual
> islet on a pancreas slide stained with H&E.  I wonder how should I do this
> as both the acinar and islets are stained with hematoxylin and eosin, and
> the adjacent blood vessels are also stained the same color as islets.
>
> Please find below example of my slides.
>
> Thank you.
>
> Creas http://n2.nabble.com/file/n4418723/624-5.jpg 
> --
> View this message in context:
> http://n2.nabble.com/islet-area-on-H-E-slide-tp4418723p4418723.html
> Sent from the ImageJ mailing list archive at Nabble.com.

--
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http://portal.gmx.net/de/go/dsl02

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[Michael Cammer-2]

Unless you have thousands of slides, just set the scale globally,
trace each islet and measure.  Sometimes manual labor is faster than
writing an automated routine, especially if you are only going to need
to do the analysis a few times.

On Tue, Jan 19, 2010 at 3:27 AM, Creas <[hidden email]> wrote:

> Dear Sir,
>
> I am a PhD student and I am trying to determine the size of each individual
> islet on a pancreas slide stained with H&E.  I wonder how should I do this
> as both the acinar and islets are stained with hematoxylin and eosin, and
> the adjacent blood vessels are also stained the same color as islets.
>
> Please find below example of my slides.
>
> Thank you.
>
> Creas http://n2.nabble.com/file/n4418723/624-5.jpg
> --
> View this message in context: http://n2.nabble.com/islet-area-on-H-E-slide-tp4418723p4418723.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>

--
Michael Cammer  *  http://coxcammer.com/
[hidden email]  or  [hidden email]
H 914-632-3044     Cell 914-309-3270

[Creas]

Many thanks for your advice.  Will give it a go.

Thank you.

Creas

--- On Wed, 20/1/10, Michael Cammer-2 [via ImageJ] <[hidden email]> wrote:

From: Michael Cammer-2 [via ImageJ] <[hidden email]>
Subject: Re: islet area on H&E slide
To: "Creas" <[hidden email]>
Received: Wednesday, 20 January, 2010, 10:39 PM

Unless you have thousands of slides, just set the scale globally,
trace each islet and measure.  Sometimes manual labor is faster than
writing an automated routine, especially if you are only going to need
to do the analysis a few times.

On Tue, Jan 19, 2010 at 3:27 AM, Creas <[hidden email]> wrote:

> Dear Sir,
>
> I am a PhD student and I am trying to determine the size of each individual
> islet on a pancreas slide stained with H&E.  I wonder how should I do this
> as both the acinar and islets are stained with hematoxylin and eosin, and
> the adjacent blood vessels are also stained the same color as islets.
>
> Please find below example of my slides.
>
> Thank you.
>
> Creas http://n2.nabble.com/file/n4418723/624-5.jpg
> --
> View this message in context: http://n2.nabble.com/islet-area-on-H-E-slide-tp4418723p4418723.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>

--
Michael Cammer  *  http://coxcammer.com/
[hidden email]  or  [hidden email]
H 914-632-3044     Cell 914-309-3270

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