This paper simply used the analyze particles command.
http://www.sciencedirect.com/science/article/pii/S0020751905002948DOI: 10.1016/j.ijpara.2005.08.013
"Swelling was video-monitored every 3 s with a Zeiss SV11 dissecting microscope (Zeiss, Göttingen, Germany) at 66×magnification, and a Retiga 1300 digital camera and IPLab software (Scanalytics, Fairfax, VA). Image analysis was accomplished with ImageJ software (National Institutes of Health, US; URL:
http://rsb.info.nih.gov/ij/) by converting images to binary and treating the oocyte as a growing sphere whose volume could be inferred from its cross-sectional area."
===========================================================================
Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
Cell: 914-309-3270 note that we do not receive messages left at 212-263-3208
http://ocs.med.nyu.edu/microscopy &
http://microscopynotes.com/-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Li, Tian
Sent: Monday, September 22, 2014 6:01 PM
To:
[hidden email]
Subject: macro help
Hello dear ImageJ users,
I'm wondering if anyone is using imageJ to monitor the water uptake in oocytes? Currently our lab switch from the old software to image J, sadly, the macro used for the old software is not compatible with imageJ, so I'm wondering if anyone can help me to convert the macro to image J compatible one, I have the written down macro, just need to use the correct syntax for image J, but I'm new to use Image J and have no clue about writing a macro, so if anyone can give me a hint, it will be greatly appreciated. I attach the macro here.
Thanks,
Tian?
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metalloid Oocyte data collection macro.txt (4K)