measuring size and velocity of droplets in flow

[Martin Ward-2]

Hi,

I have began a series of experiments that involve the production of  
droplets in a carrier fluid.  It is important that I can measure their  
size (diameter) and their velocity as they move in a linear flow.

Currently I am producing a stack of images and then measuring these  
values manually.  I would be interested to find out if there is a  
specific plugin that I might be able to use for my purpose.  I have  
been searching online but have not found anything suitable so far.

An example of the typical stack I am working with can be found at the  
following Google drive location >>>  
https://drive.google.com/file/d/0B66eKr8PljEBVDVNaF9WQVZKc2s/view?usp=sharing

I don't know if it would be possible to include a selection of region  
of interest prior to the analysis, i.e. a box in the center of the  
screen for example that would allow the debris/residue seen at either  
end of the channel to be ignored?

For reference the channel width (channel runs horizontally along the  
frame) is 100 micron wide in the vertical direction, and the image  
size is 2040*800 pixels.


Regards,
Martin.

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[jerie]

Hi Martin,

by the sounds of it,  one of the kymograph/time-space plot implementations
should work. I did not manage to open your example file.

Cheers, Jens

http://br.linkedin.com/pub/jens-rietdorf/6/4a3/189/
 Am 06.05.2015 13:17 schrieb "Martin Ward" <[hidden email]>:

> Hi,
>
> I have began a series of experiments that involve the production of
> droplets in a carrier fluid.  It is important that I can measure their size
> (diameter) and their velocity as they move in a linear flow.
>
> Currently I am producing a stack of images and then measuring these values
> manually.  I would be interested to find out if there is a specific plugin
> that I might be able to use for my purpose.  I have been searching online
> but have not found anything suitable so far.
>
> An example of the typical stack I am working with can be found at the
> following Google drive location >>>
> https://drive.google.com/file/d/0B66eKr8PljEBVDVNaF9WQVZKc2s/view?usp=sharing
>
> I don't know if it would be possible to include a selection of region of
> interest prior to the analysis, i.e. a box in the center of the screen for
> example that would allow the debris/residue seen at either end of the
> channel to be ignored?
>
> For reference the channel width (channel runs horizontally along the
> frame) is 100 micron wide in the vertical direction, and the image size is
> 2040*800 pixels.
>
>
> Regards,
> Martin.
>
> --
> The University of Edinburgh is a charitable body, registered in
> Scotland, with registration number SC005336.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html