merging images

[Santosh M]

hi

I have taken images using different filters under fluorescence microscope
(Purpose: double Immunohistochemistry)
How can I merge those images in one image by using Image J.

Thanks for any help

regards

Santosh

[Jacqueline Ross]

Hi Santosh,

Open the images that you want to merge. Then just go to Image - Color -
Merge Channels. Use the drop-down boxes to select the image you want for
each colour channel and proceed. You can choose to keep the source
images or not.

If you haven't matched the intensities well when capturing, you can
adjust those intensities first under Image - Adjust - Window/Level.

Kind regards,

Jacqui

Jacqueline Ross

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Santosh M
Sent: Thursday, 30 April 2009 4:46 p.m.
To: [hidden email]
Subject: merging images

hi

I have taken images using different filters under fluorescence
microscope
(Purpose: double Immunohistochemistry)
How can I merge those images in one image by using Image J.

Thanks for any help

regards

Santosh

[Santosh M]

Dear Jacqueline Ross

Thanks for your help

regards

Santosh

2009/4/30 Jacqui Ross <[hidden email]>

> Hi Santosh,
>
> Open the images that you want to merge. Then just go to Image - Color -
> Merge Channels. Use the drop-down boxes to select the image you want for
> each colour channel and proceed. You can choose to keep the source
> images or not.
>
> If you haven't matched the intensities well when capturing, you can
> adjust those intensities first under Image - Adjust - Window/Level.
>
> Kind regards,
>
> Jacqui
>
> Jacqueline Ross
>
> Biomedical Imaging Microscopist
> Biomedical Imaging Research Unit
> School of Medical Sciences
> Faculty of Medical & Health Sciences
> The University of Auckland
> Private Bag 92019
> Auckland, NEW ZEALAND
>
> Tel: [phone] Ext 87438
> Fax: [phone]
>
> http://www.fmhs.auckland.ac.nz/sms/biru/
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> Santosh M
> Sent: Thursday, 30 April 2009 4:46 p.m.
> To: [hidden email]
> Subject: merging images
>
> hi
>
> I have taken images using different filters under fluorescence
> microscope
> (Purpose: double Immunohistochemistry)
> How can I merge those images in one image by using Image J.
>
> Thanks for any help
>
> regards
>
> Santosh
>