Hi Dave,
We have recently acquired a microscope with a motorized stage. This means
> that we can now acquire multi-position, multi-channel, multi-Z time lapse
> data. Is anyone thinking about adding another degree of freedom to Loci,
> Image5D or stack manipulation plugins to allow for this extra variable?
> Dave
>
Good idea. Actually, Bio-Formats does support multi-position data, in the
form of multiple image series. When importing the data, you should get a
dialog box asking which of the fields/positions you wish to open, with each
field opening into its own image window. We have posted an example
multi-field dataset called MF-2CH-Z-T online at:
http://www.loci.wisc.edu/visbio/data.htmlAs always, please let us know if you have any questions, suggestions or
problems with it.
Cheers,
Curtis
On Tue, Feb 17, 2009 at 8:39 AM, David Knecht <
[hidden email]>wrote:
> We have recently acquired a microscope with a motorized stage. This means
> that we can now acquire multi-position, multi-channel, multi-Z time lapse
> data. Is anyone thinking about adding another degree of freedom to Loci,
> Image5D or stack manipulation plugins to allow for this extra variable?
> Dave
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
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