ned help with new macro for backgournd subtraction from ROIs

[sean_incali]

I know we have a great macro for background subtraction given a single ROI.

But what i need is a way to subtract the background from the average intensity
calculated from multiple ROIs.

Basically what I need is a way to calculate the average intensity of multiple
ROIs in a given image. (maybe 3 ROIs) Then use *that* average of the
intensities to background subtract the entire image.

IS that possible? if so, how?  I could really use some help here. Any and all
comments are welcome.

thanks all in advance

sean

[Tony Collins-2]

Hi Sean,

If you hold the shift-key down when you select your ROI you can add
multiple ROIs. Does this work for you?

Hoiwever, if your background is uneven, you may want to try the core
background-subtraction function instead of subtracting a single value
across the image. (Process>Background subtraction)

Regards,

Tony

sean wrote:

> I know we have a great macro for background subtraction given a single ROI.
>
> But what i need is a way to subtract the background from the average intensity
> calculated from multiple ROIs.
>
> Basically what I need is a way to calculate the average intensity of multiple
> ROIs in a given image. (maybe 3 ROIs) Then use *that* average of the
> intensities to background subtract the entire image.
>
> IS that possible? if so, how?  I could really use some help here. Any and all
> comments are welcome.
>
> thanks all in advance
>
> sean

--
Tony Collins, Ph.D.
Facility Manager
Wright Cell Imaging Facility
Toronto Western Research Institute
13-407 McLaughlin Pavilion
399 Bathurst Street
Toronto, ON. M5T 2S8
tel. [phone] fax: [phone]
http://www.uhnresearch.ca/wcif

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