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Hi All,
Apologies if this is already covered elsewhere obvious and I have missed it. I am a beginner in this so quite possible.
I need to quantify a granular neurite staining which also extends to soma. Is it possible to create neurite traces in one channel, i.e. on beta-3 tubulin staining, using something like NeuroJ and then using that as a selected area to quantify these puncta , or any staining intensity, in another channel? If so, how does one normalise the data between different experiments?
Many thanks!
B
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