Dear all,
hereby an offer for an open position within our lab.
If someone might be interested you may reply to my mail or contact
Manuel Théry as stated below.
Grenoble, 16 th of December, 2012
The team "Physics of the Cytoskeleton and Morphogenesis" of the
CEA (Grenoble, France) will hire as soon as possible someone skilled
in microscopy and image analysis for a period of 18 months.
Our team works on the development of new technological devices to
control cell architecture (1), and analyze the cell polarization
process (2).
For more details about the work in our team:
http://sites.google.com/site/manuelthery/ The research project is a partnership with the company CYTOO,
which produces micropatterned slides for cell biology analyses. The
project is funded by the OSEO program supporting innovations for
industrial applications. The purpose of the project is to engineer new
devices in order to constrain multicellular arrangements in
reproducible conformations. These devices will allow the analysis in
vitro of cell phenotypes in complex, organized, multicellular context
mimicking genuine tissue architectures.
Our team has recently developed a new micropatterning method
based on the scanning of a pulsed-laser on a glass surface coated with
poly-ethyleneglycol. This technique allows the rapid production of any
kind of micropattern shapes with sub-micrometric resolution. It can be
used to design and improve the shapes of the micropatterns
constraining multicellular structures.
The tasks during the 18 monthes will be the following:
* Votre navigateur ne gère peut-être pas l'affichage de cette
image. develop a user-friendly plug-in to turn a given shape into
controled displacements of the laser, and to repeat these displacement
over a 10x10 mm glass slide. We already use galvanometric mirror to
control laser displacement and a laser-based autofocus system to find
the right focus all over the glass slide.
* improve our automated image acquisition process in order to scan
a 20x20 mm slide and record specifically the multicellular structures
made of the expected cell number.
Votre navigateur ne gère peut-être pas l'affichage de cette image.
* improve our image analysis tool to detect cell compartments and
register their positions. Simple image segmentation and filtering have
been used so far in our team to detect cell nucleus, centrosome, Golgi
apparatus and so on but need to be improved to become more robust and
overcome variations in image quality. These simple image analysis
steps need also to be adapted to process batches of image stacks.
For more details about the project and the work to be performed :
manuel.thery(at)cea.fr
1- Tseng et al. PNAS, 2012, in press; Reymann et al., Nature
Materials, 2010; Thery et al., Nature, 2007;
2- Pitaval et al., Journal of Cell Biology, 2010; Thery et al. PNAS,
2006; Thery et al. Nature Cell Biology 2005.
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