(no subject)

[OBEID Patricia 154904]

Hello everyone,
I try to quantify immunofluorescence stainings. For some cells, the marking is crown or central or sometimes both ...
I have the mask of the cells and I would define from this mask two areas: one in the external ring, the other in the center.
Is it possible from the mask to create a ring that start from the outside and would extend from "x" pixels inside?
For the center can we retrieve information from the central point of the mask and create a circle of radius "y" pixels there?
Thank you all for your help
Cordially
Patricia

_______________________________________
Patricia OBEÏD
Animateur Informatique Biomics
CEA Grenoble
DSV / iRTSV / BGE U1038/ Biomics / Bât 4020
17 rue des Martyrs
38054 GRENOBLE Cedex 9

Email : [hidden email]
Tél : [phone]
Fax : [phone]
http://dsv.cea.fr/dsv/irtsv/Pages/BGE/Biomics/Accueil.aspx

https://twitter.com/BiomicsLab<https://webmail.cea.fr/owa/redir.aspx?C=bRI7b13yVUCyl4Teeaj0LP1thcSl79EIQmY_8bFerByjYaJbW5sHRoukVNwHOIjgSG9lrQnp9wk.&URL=https%3a%2f%2ftwitter.com%2fBiomicsLab>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Laure Twyffels]

Hi Patricia,

A simple proposition: once you have created your "total" mask, you can duplicate it. On the duplicated image, you can erode the mask x times (process/binary/erode). You can then subtract the small mask from the total mask to get your "crown" mask (process/image calculator).

Best,

Laure

[OBEID Patricia 154904]

very good trick!
thank you Laure
Patricia
________________________________________
De : ImageJ Interest Group [[hidden email]] de la part de Laure Twyffels [[hidden email]]
Envoyé : mardi 12 mai 2015 09:28
À : [hidden email]
Objet : Re: (no subject)

Hi Patricia,

A simple proposition: once you have created your "total" mask, you can
duplicate it. On the duplicated image, you can erode the mask x times
(process/binary/erode). You can then subtract the small mask from the total
mask to get your "crown" mask (process/image calculator).

Best,

Laure



--
View this message in context: http://imagej.1557.x6.nabble.com/no-subject-tp5012805p5012812.html
Sent from the ImageJ mailing list archive at Nabble.com.

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html