nuclei intensity in a timelapse

[F Javier Díez Guerra]

Hello,

I want to measure the average intensity of cell nuclei from a
timelapse (aprox 400 frames) with 2-3 cells expressing a GFP fusion
that enters and leaves the nucleus. The cells are moving and the
nuclei also move and change shape.

In the end, each frame should be thresholded to obtain 2-3 ROIs
outlining cell nuclei where average intensity could be measured. Any
idea on how to automate this task?

Thanks,

[Sami Badawi-2]

Hi Javier,

I have a little OSS project ShapeLogic that is investigating use of
declarative programming techniques in image processing.
My next code example is an automatic color and gray scale particle
counter. If that is successful I am planning to start working on a
code example very similar to what you describe.

I don't know if and when I can do it, but if nobody else has any
suggestions I will let you know if I am successful.

-Sami Badawi
http://www.shapelogic.org

On Fri, Mar 14, 2008 at 10:49 AM, F Javier Diez Guerra
<[hidden email]> wrote:

> Hello,
>
>  I want to measure the average intensity of cell nuclei from a
>  timelapse (aprox 400 frames) with 2-3 cells expressing a GFP fusion
>  that enters and leaves the nucleus. The cells are moving and the
>  nuclei also move and change shape.
>
>  In the end, each frame should be thresholded to obtain 2-3 ROIs
>  outlining cell nuclei where average intensity could be measured. Any
>  idea on how to automate this task?
>
>  Thanks,
>