number of results to excel?

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number of results to excel?

Oliver Dick
Dear List-members,

I want to analyze images with cells that show different intensity for a
certain nuclear staining. Currently I set the threshold manually and use
the analyze particles feature of ImageJ to count either all or the cells
with the high intensity. From the results table I only need the last
number because I am only interested in the number of cells with a
certain intensity-range.

Unfortunately I have a lot of images to analyze -- meaning open them all
twice, running the threshold/analyze particle thing and then I just note
the number of cells in excel.

Is there a way to tell imageJ to open several images and perform on each
the procedure and then to transfer the number of cells to an excel document?

I have tried to use the excel writer, but this gives me always the
complete results table and not only the last number.

Any help or suggestions are appreciated.

Oliver

--
Dr. Oliver Dick
Neurobiologie
Im Neuenheimer Feld 364
69120 Heidelberg

Tel.: ++49/(0)6221/548329
Fax.: ++49/(0)6221/546700
Email.: [hidden email]
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Re: number of results to excel?

Arne Seitz-2
Dear Oliver,

the attached makro should more or less do what you want to do (if I got it
correct). If you still have questions you can also contact me off-list.


requires("1.33s");
dir1 = getDirectory("Choose Source Directory ");
list = getFileList(dir1);
setBatchMode(true);
  for (i=0; i<list.length; i++) {
    showProgress(i+1, list.length);
    open(dir1+list[i]);
    setAutoThreshold();
    run("Analyze Particles...", "size=0-Infinity circularity=0.00-1.00
show=Nothing display exclude clear summarize record");
    close();        
       
  }

Cheers Arne

____________________________________________________
 
Arne Seitz, Scientific Officer
Advanced Light Microscopy Facility
EMBL Heidelberg
 
+49 6221 387 8467

-----Ursprüngliche Nachricht-----
Von: ImageJ Interest Group [mailto:[hidden email]] Im Auftrag von
Oliver Dick
Gesendet: Donnerstag, 16. August 2007 15:30
An: [hidden email]
Betreff: number of results to excel?

Dear List-members,

I want to analyze images with cells that show different intensity for a
certain nuclear staining. Currently I set the threshold manually and use
the analyze particles feature of ImageJ to count either all or the cells
with the high intensity. From the results table I only need the last
number because I am only interested in the number of cells with a
certain intensity-range.

Unfortunately I have a lot of images to analyze -- meaning open them all
twice, running the threshold/analyze particle thing and then I just note
the number of cells in excel.

Is there a way to tell imageJ to open several images and perform on each
the procedure and then to transfer the number of cells to an excel document?

I have tried to use the excel writer, but this gives me always the
complete results table and not only the last number.

Any help or suggestions are appreciated.

Oliver

--
Dr. Oliver Dick
Neurobiologie
Im Neuenheimer Feld 364
69120 Heidelberg

Tel.: ++49/(0)6221/548329
Fax.: ++49/(0)6221/546700
Email.: [hidden email]