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Dear List-members,
I want to analyze images with cells that show different intensity for a certain nuclear staining. Currently I set the threshold manually and use the analyze particles feature of ImageJ to count either all or the cells with the high intensity. From the results table I only need the last number because I am only interested in the number of cells with a certain intensity-range. Unfortunately I have a lot of images to analyze -- meaning open them all twice, running the threshold/analyze particle thing and then I just note the number of cells in excel. Is there a way to tell imageJ to open several images and perform on each the procedure and then to transfer the number of cells to an excel document? I have tried to use the excel writer, but this gives me always the complete results table and not only the last number. Any help or suggestions are appreciated. Oliver -- Dr. Oliver Dick Neurobiologie Im Neuenheimer Feld 364 69120 Heidelberg Tel.: ++49/(0)6221/548329 Fax.: ++49/(0)6221/546700 Email.: [hidden email] |
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Dear Oliver,
the attached makro should more or less do what you want to do (if I got it correct). If you still have questions you can also contact me off-list. requires("1.33s"); dir1 = getDirectory("Choose Source Directory "); list = getFileList(dir1); setBatchMode(true); for (i=0; i<list.length; i++) { showProgress(i+1, list.length); open(dir1+list[i]); setAutoThreshold(); run("Analyze Particles...", "size=0-Infinity circularity=0.00-1.00 show=Nothing display exclude clear summarize record"); close(); } Cheers Arne ____________________________________________________ Arne Seitz, Scientific Officer Advanced Light Microscopy Facility EMBL Heidelberg +49 6221 387 8467 -----Ursprüngliche Nachricht----- Von: ImageJ Interest Group [mailto:[hidden email]] Im Auftrag von Oliver Dick Gesendet: Donnerstag, 16. August 2007 15:30 An: [hidden email] Betreff: number of results to excel? Dear List-members, I want to analyze images with cells that show different intensity for a certain nuclear staining. Currently I set the threshold manually and use the analyze particles feature of ImageJ to count either all or the cells with the high intensity. From the results table I only need the last number because I am only interested in the number of cells with a certain intensity-range. Unfortunately I have a lot of images to analyze -- meaning open them all twice, running the threshold/analyze particle thing and then I just note the number of cells in excel. Is there a way to tell imageJ to open several images and perform on each the procedure and then to transfer the number of cells to an excel document? I have tried to use the excel writer, but this gives me always the complete results table and not only the last number. Any help or suggestions are appreciated. Oliver -- Dr. Oliver Dick Neurobiologie Im Neuenheimer Feld 364 69120 Heidelberg Tel.: ++49/(0)6221/548329 Fax.: ++49/(0)6221/546700 Email.: [hidden email] |
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