open only part of an image

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open only part of an image

mikeadams709
I am trying to open (put in memory) only part of a large image.  The image I'm trying to use is a 2D image approximately 30Gb and is composed of many tiles.  I do not have access to the individual tiles, I only have access to the finally stitched image.  I need to do some automated cropping on this image but I first need to be able to work with this size of image.  

recap:
 I need to access only part of an image file at a time.  it is an Imaris image file (.ims).  Can this be done through imageJ?

Thanks in advance

Mike
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Re: open only part of an image

Melissa Linkert-2
Hi Mike,

> I am trying to open (put in memory) only part of a large image.  The image I'm trying to use is a 2D image approximately 30Gb and is composed of many tiles.  I do not have access to the individual tiles, I only have access to the finally stitched image.  I need to do some automated cropping on this image but I first need to be able to work with this size of image.  
>
> recap:
>  I need to access only part of an image file at a time.  it is an Imaris image file (.ims).  Can this be done through imageJ?

Yes, this can be done if you are using the Bio-Formats importer plugin
(http://loci.wisc.edu/software/bio-formats) to open the file.
Just select the "Crop on import" option in the "Bio-Formats Import Options"
window, then enter the XY coordinate of the upper-left corner along with the
width and height of the part of the image that you want to view.

Regards,
-Melissa

On Mon, Jan 02, 2012 at 01:59:33AM -0500, Mike Adams wrote:
> I am trying to open (put in memory) only part of a large image.  The image I'm trying to use is a 2D image approximately 30Gb and is composed of many tiles.  I do not have access to the individual tiles, I only have access to the finally stitched image.  I need to do some automated cropping on this image but I first need to be able to work with this size of image.  
>
> recap:
>  I need to access only part of an image file at a time.  it is an Imaris image file (.ims).  Can this be done through imageJ?
>
> Thanks in advance
>
> Mike