overlaying a stack

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overlaying a stack

ergefan
hi imagej-ers,

does anyone know how to do an overlay of a stack containing multiple images? the ADD function in Image Calculator only allows overlay of 2 images at a time.

Please help. thanks a lot!
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Re: overlaying a stack

Michael Schmid
Hi,

you can try the Blend Images Plugin. It can calculate
   (1 - x) * CurrentImage + x * Image2
or
   CurrentImage + x * Image2
with user defined 'x'.

It works also for composite images and stacks (if they have the same  
size).

http://rsb.info.nih.gov/ij/plugins/blend-images.html

Michael
________________________________________________________________

On 3 May 2010, at 17:23, Kai Fan wrote:

> hi imagej-ers,
>
> does anyone know how to do an overlay of a stack containing  
> multiple images? the ADD function in Image Calculator only allows  
> overlay of 2 images at a time.
>
> Please help. thanks a lot!
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Re: overlaying a stack

Gary Sellani
If the images were all of equal weight, wouldn't it be equivalent to use
the
image->stacks->zproject->average intensity
?

That is, do you use this blend images plugin only for unequal weighting?


On 5/3/2010 8:56 AM, Michael Schmid wrote:

> Hi,
>
> you can try the Blend Images Plugin. It can calculate
> (1 - x) * CurrentImage + x * Image2
> or
> CurrentImage + x * Image2
> with user defined 'x'.
>
> It works also for composite images and stacks (if they have the same size).
>
> http://rsb.info.nih.gov/ij/plugins/blend-images.html
>
> Michael
> ________________________________________________________________
>
> On 3 May 2010, at 17:23, Kai Fan wrote:
>
>> hi imagej-ers,
>>
>> does anyone know how to do an overlay of a stack containing multiple
>> images? the ADD function in Image Calculator only allows overlay of 2
>> images at a time.
>>
>> Please help. thanks a lot!
>
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Re: overlaying a stack

Michael Schmid
Hi Gary, everyone else,

maybe I misunderstood the question; I understood 'overlay' as 'add  
something with some weight to all images'.
If the question was about an operation on all images of one stack,  
you are right, the answer would be image->stacks->zproject

Michael
________________________________________________________________

On 4 May 2010, at 08:13, gary wrote:

> If the images were all of equal weight, wouldn't it be equivalent  
> to use the
> image->stacks->zproject->average intensity
> ?
>
> That is, do you use this blend images plugin only for unequal  
> weighting?
>
>
> On 5/3/2010 8:56 AM, Michael Schmid wrote:
>> Hi,
>>
>> you can try the Blend Images Plugin. It can calculate
>> (1 - x) * CurrentImage + x * Image2
>> or
>> CurrentImage + x * Image2
>> with user defined 'x'.
>>
>> It works also for composite images and stacks (if they have the  
>> same size).
>>
>> http://rsb.info.nih.gov/ij/plugins/blend-images.html
>>
>> Michael
>> ________________________________________________________________
>>
>> On 3 May 2010, at 17:23, Kai Fan wrote:
>>
>>> hi imagej-ers,
>>>
>>> does anyone know how to do an overlay of a stack containing multiple
>>> images? the ADD function in Image Calculator only allows overlay  
>>> of 2
>>> images at a time.
>>>
>>> Please help. thanks a lot!
>>