particle analysis in bubble motion experiments

[Marcel Krzan]

Dear Friends,

I use the ImageJ Macros in my reseach work. I already prepared few Macros,
which analysed the bubble motion in various surfacxtant solution.

Till now I used the particle analysis function and analysed the moving of
center of the mass. However now in my experiments I have a strong pulsation
of the bubble shape which influenced the values of the bubble local
velocities.

Therefore I need to find the coordinates (X,Y) of the points on the bubble
TOP and BOTTOM

Can you help me? In which way could I modify the Particle analysis function
to obtain those data.

Regards
Marcel Krzan

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Jerome Mutterer-3]

Dear Marcel,
You should add the "Bounding Rectangle" measurement to the list of measured
parameters under Analyze>Set Measurements...
BY will be the top of an object, and BY+Height will give you the bottom
coordinate.
For your X coordinate, you could use BX+(Width/2)
Sincerely,
Jerome.

On 14 October 2014 14:44, Marcel Krzan <[hidden email]> wrote:

> Dear Friends,
>
> I use the ImageJ Macros in my reseach work. I already prepared few Macros,
> which analysed the bubble motion in various surfacxtant solution.
>
> Till now I used the particle analysis function and analysed the moving of
> center of the mass. However now in my experiments I have a strong pulsation
> of the bubble shape which influenced the values of the bubble local
> velocities.
>
> Therefore I need to find the coordinates (X,Y) of the points on the bubble
> TOP and BOTTOM
>
> Can you help me? In which way could I modify the Particle analysis function
> to obtain those data.
>
> Regards
> Marcel Krzan
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
Jerome Mutterer
CNRS - Institut de biologie moléculaire des plantes
12, rue du Général Zimmer
67084 Strasbourg Cedex
T 0367155339
www.ibmp.cnrs.fr

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