Hi,
On Oct 2, 2011, at 8:13 AM, Marcel Krzan wrote:
> Hi,
>
> I would like to ask You about the particle analysis function. Is it
> possible to measure the length of the particle perimeter?
>
Perimeter is an estimate of the path length around the outer edge of a particle. What do you mean by "length of the particle perimeter"?
> The other question is also connected with this (particle analysis)
> measurement. The function give us as a result the total particle area.
> It is good, but I need moe precise information.
> Is it possible to measure particle sizes line by line. Maybe it is
> difficult to explain, but - I have spherical or elipsoidal bubbles rising
> in the liquids. Bubbles are axi-symentric. Our data (photos) of course
> are only 2D -what means that the images of the bubbles give us 2D
> infomations. I would like to reconstruct the bubble 3D image based on the
> 2D images. And therefore I need to find the information about the bubble
> horizontal sizes exactly line by line from the bubble top till the
> bottom. It will be great if I could find i.e. the coordinates of the
> points on the bubble perimenter ...
> If You can help Me - it will be great.
> Thanks
> Marcel Krzan
Sieracki, Viles and Webb used 2d particle analysis to estimate volume of cells. Bubbles tend to conserve surface area so to it seems like it ought to be a safe bet to use the ideas in their paper on bubbles. I have posted a copy of the paper here...
http://dl.dropbox.com/u/8433654/Sieracki.1989.pdf.
Getting the coordinates of the perimeter pixels is not difficult, but you don't say if you are working in the macro language, javascript or in java as a plugin.
Cheers,
Ben
Locked
