plot profile behaviour

[Christian Liebig]

Dear all,

I observe the following and I don't know why this happens:
when I draw a segmented line and use "Plot Profile" from the "Analyze"
menu I get a profile plot along this line, which is what I want.
However, if I do the same thing with a line width other than 1 my
segmented line is transformed into a curve and I don't want this.
Any ideas why this is? It does not happen if I use the RGB profiler
plugin instead.

Kind regards,

Christian



--



Christian Liebig, PhD

FILM - Facility for Imaging by Light Microscopy
- Facility Assistant -
Sir Alexander Fleming Building, desk 403
Imperial College London / South Kensington
Exhibition Road
London SW7 2AZ
UK

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Website: http://imperial.ac.uk/imagingfacility

[Wayne Rasband]

> Dear all,
>
> I observe the following and I don't know why this happens:
> when I draw a segmented line and use "Plot Profile" from the  
> "Analyze" menu I get a profile plot along this line, which is what I  
> want. However, if I do the same thing with a line width other than 1  
> my segmented line is transformed into a curve and I don't want this.
> Any ideas why this is? It does not happen if I use the RGB profiler  
> plugin instead.

The Analyze>Plot Profile command averages across the line when the  
line width is greater than one. It does this by straightening the line  
(Edit>Selection>Straighten) and then profiling. The line is converted  
into a curve because the Straighten command fits a spline to the line.  
The RGB Profiler plugin does not average across lines that are wider  
than one.

-wayne