Hi Thomas,
sorry, no direct answer, but maybe an alternative:
- combine (OR) all the ROIs in the ROI manager
- use the 'Fit Polynomial' plugin:
https://imagejdocu.tudor.lu/plugin/filter/fit_polynomial/startThis plugin has a different strategy concerning the background level,
however: It puts the average of the ROI to 128 for 8-bit images and
32768 for 16-bit images, so it may result in overflow for the forground.
If you have 8-bit images, or 16-bit images usuing the full 16 bits, you
will have to convert to 16 or 32 bits, respectively, before applying the
background.
Another way to avoid saturation is setting the B&C as descibed on the
plugin page and checking "Shift Values to Display Range".
For 8-bit and 16-bit images it will also apply a calibration, such that
the background level is zero.
Of course, you can record these steps with the macro recorder and then
create a macro for it.
Michael
________________________________________________________________
On 04.05.20 20:04, Thomas Fischer wrote:
> Hi,
>
> the link to download this plugin on the following page does not work
>
>
https://imagejdocu.tudor.lu/plugin/filter/nonuniform_background_removal/start>
> is there another location?
>
> Thank you
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