problem with TIF from ImageProPlus

[Cammer, Michael]

We have images from a Leica microscope set up with ImageProPlus that we cannot open into ImageJ.  Any help appreciated.

They are RGB color 1392 X 1040 pixels, file length in range of 6,523,992 or 6,527,624 bytes.


They do open in Photoshop, and when we save them as TIF they get larger by about 30% (maybe they were compressed?).  Then when we open them in ImageJ they are 15 or 16 bits per channel.


Any ideas whether we can open them into ImageJ or should we find a workaround, like relearning how to do simple automation using Photoshop?


Thank you!

Michael Cammer, Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016
C: 914-309-3270  [hidden email]<mailto:[hidden email]>    http://microscopynotes.com/
https://med.nyu.edu/research/research-resources/scientific-cores-shared-resources/microscopy-laboratory

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[ctrueden]

Hi Michael,

Could you please post a sample non-working image somewhere public?

Regards,
Curtis

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Curtis Rueden
LOCI software architect - https://loci.wisc.edu/software
ImageJ2 lead, Fiji maintainer - https://imagej.net/User:Rueden
Did you know ImageJ has a forum? http://forum.imagej.net/


On Mon, Nov 6, 2017 at 7:58 PM, Cammer, Michael <[hidden email]>
wrote:

> We have images from a Leica microscope set up with ImageProPlus that we
> cannot open into ImageJ.  Any help appreciated.
>
> They are RGB color 1392 X 1040 pixels, file length in range of 6,523,992
> or 6,527,624 bytes.
>
>
> They do open in Photoshop, and when we save them as TIF they get larger by
> about 30% (maybe they were compressed?).  Then when we open them in ImageJ
> they are 15 or 16 bits per channel.
>
>
> Any ideas whether we can open them into ImageJ or should we find a
> workaround, like relearning how to do simple automation using Photoshop?
>
>
> Thank you!
>
> Michael Cammer, Research Scientist, DART Microscopy Laboratory
> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY
> 10016
> C: 914-309-3270  [hidden email]<mailto:[hidden email].
> edu>    http://microscopynotes.com/
> https://med.nyu.edu/research/research-resources/scientific-
> cores-shared-resources/microscopy-laboratory
>
> ------------------------------------------------------------
> This email message, including any attachments, is for the sole use of the
> intended recipient(s) and may contain information that is proprietary,
> confidential, and exempt from disclosure under applicable law. Any
> unauthorized review, use, disclosure, or distribution is prohibited. If you
> have received this email in error please notify the sender by return email
> and delete the original message. Please note, the recipient should check
> this email and any attachments for the presence of viruses. The
> organization accepts no liability for any damage caused by any virus
> transmitted by this email.
> =================================
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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