problem with hyperstacks

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problem with hyperstacks

Volker Baecker
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Hello,

I encounter the following problem using ImageJ 1.43l and 1.42q.
- - I create a hyperstack with the "Merge Channels" command
- - I open the b&c adjuster
- - I change the brightness of the first channel
- - I switch to the second channel
- - I try to change the brightness but there the brightness of the first
channel is changed instead.

It works ok, when I create the hyperstack using "images to stack" and
"stack to hyperstack" instead.

However when I save the hyperstack image as tif and reload it the
problem is there again.

Here are two images for which this happens:

http://www.mri.cnrs.fr/mriwiki/uploads/rhod_3.tif
http://www.mri.cnrs.fr/mriwiki/uploads/dapi_3.tif

Does anyone encounter the same problem?

Best regards,
Volker Bäcker
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Re: problem with hyperstacks

Wayne Rasband
 > Hello,
 >
 > I encounter the following problem using ImageJ 1.43l and 1.42q.
 > - - I create a hyperstack with the "Merge Channels" command
 > - - I open the b&c adjuster
 > - - I change the brightness of the first channel
 > - - I switch to the second channel
 > - - I try to change the brightness but there the brightness of
 > the first channel is changed instead.
 >
 > It works ok, when I create the hyperstack using "images to stack"
 >  and "stack to hyperstack" instead.
 >
 > However when I save the hyperstack image as tif and reload
 > it the problem is there again.
 >
 > Here are two images for which this happens:
 >
 > http://www.mri.cnrs.fr/mriwiki/uploads/rhod_3.tif
 > http://www.mri.cnrs.fr/mriwiki/uploads/dapi_3.tif
 >
 > Does anyone encounter the same problem?
 >
 > Best regards,
 > Volker Bäcker

This bug is fixed in the 1.43m daily build, thanks to a fix provided by
Volker. The problem occurs when changing channels by dragging the
channel selection slider. It does not occur when changing channels by
clicking on the slider, by clicking on the arrows, by using the scroll
wheel, or by using the "<", ">", left arrow or right arrow keyboard
shortcuts.

-wayne