put multiple arrows in one video

[inesica]

Hello,

I am trying to add arrows on a video on imagej. when i use the arrow tool
plug-in, i can add one arrow on the video, but when i add another one, the
first one dissapears. i tried saving the video with the first arrow, and
then adding the second arrow on the saved video containing the first arrow,
but still, the first arrow dissapears. is there a key i should press (like
ctrl+d for text) that saves the arrow on the video?

Thank you very much for your help and have a nice day!



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[Cammer, Michael]

Perhaps you could flatten the image (Image > Overlay  > Flatten) and save a temp version each time you add a new element.


Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016

[hidden email]<mailto:[hidden email]>  http://nyulmc.org/micros  http://microscopynotes.com/

Voice direct only, no text or messages:  1-914-309-3270 and 1-646-501-0567



________________________________
From: inesica <[hidden email]>
Sent: Monday, May 28, 2018 5:37:48 AM
To: [hidden email]
Subject: put multiple arrows in one video

Hello,

I am trying to add arrows on a video on imagej. when i use the arrow tool
plug-in, i can add one arrow on the video, but when i add another one, the
first one dissapears. i tried saving the video with the first arrow, and
then adding the second arrow on the saved video containing the first arrow,
but still, the first arrow dissapears. is there a key i should press (like
ctrl+d for text) that saves the arrow on the video?

Thank you very much for your help and have a nice day!



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[Wayne Rasband-2]

> On May 28, 2018, at 5:37 AM, inesica <[hidden email]> wrote:
>
> Hello,
>
> I am trying to add arrows on a video on imagej. when i use the arrow tool
> plug-in, i can add one arrow on the video, but when i add another one, the
> first one dissapears. i tried saving the video with the first arrow, and
> then adding the second arrow on the saved video containing the first arrow,
> but still, the first arrow dissapears. is there a key i should press (like
> ctrl+d for text) that saves the arrow on the video?

For each error, press ctrl+b (Image>Overlay>Add Selection). You will need to first uncheck “Set stack positions” in Image>Overlay>Overlay Options if you want the arrows to appear on every video frame. The arrows will be saved with the video when you save in TIFF format. The overlay will be outomatically flattened (Image>Ovelay>Flatten) if you save in AVI (File>Save As>AVI) or GIF (File>Save As>Gif) format.

-wayne


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>

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[Krs5]

A while ago I wrote a little macro that allows you to put ROIs including arrows onto a time series and allows you to  set the start and end frame for each of the selections, see Image annotation macro at https://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/AIF/software-1/imagej-macros

Best wishes

Kees


Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
www.le.ac.uk/advanced-imaging-facility


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of inesica
Sent: 28 May 2018 10:38
To: [hidden email]
Subject: put multiple arrows in one video

Hello,

I am trying to add arrows on a video on imagej. when i use the arrow tool plug-in, i can add one arrow on the video, but when i add another one, the first one dissapears. i tried saving the video with the first arrow, and then adding the second arrow on the saved video containing the first arrow, but still, the first arrow dissapears. is there a key i should press (like
ctrl+d for text) that saves the arrow on the video?

Thank you very much for your help and have a nice day!



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