Dear Rona,
There is a plug-in for ImageJ crafted by Dr Pawel Znojek, which
essentially aims to do this.
He constructed it for DAPI and a fluorescent antibody to a DNA repair
protein.
You should be able to adapt this easily for your purposes.
The plugin is available from the following site:
http://www.pzfociez.com/ .
I would recommend that you should add a plugin constructed by Gabriel
Landini, at the University of Birmingham, England, which helps considerably.
This you can find on the ImageJ site.
If you proceed down this path, I should be very grateful to you if you
could let me know how things develop for you.
With best wishes,
Sydney
On 12/03/2013 09:20, rona baron wrote:
> Hi all,
>
> I stained tissues with antibodies specific for neurons (MAP-2) and with
> hematoxylin which stains all the nucleus population, than imaged it with
> light microscope.
> Is it possible to distinguish between the brown staining for neurons and
> the purple staining for the nucleus?
> I would like to quantify the number of brown particles that I have out of
> the total purple particles....
>
>
> Thanks,
> Rona
>
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> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
--
Professor Sydney Shall,
Department of Haematological Medicine,
King's College London,
Medical School,
123 Coldharbour Lane,
LONDON SE5 9NU,
Tel & Fax: +44 (0)207 848 5902,
E-Mail: sydney.shall,
[correspondents outside the College should add; @kcl.ac.uk]
www.kcl.ac.uk
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