hi
I am new to imageJ, and would like to understand the different options of measuring flourescence in single cell images from confocal microscope.
I am trying to quantify diffrences in:
1. endocytosis of flourescent transferrin or a fluorescent endocytosis probe FM 1-43,
2. inhibition of clathrin by shRNA - I did immunocytochemistry with anti clathrin ab and then anti mouse - cy5.
the images were split from RGB, turned into 8 bit if needed, and thresholded. I selected each cell and
then i chose the following from "Set Measurments"menu: Area, Integrated density,Area fraction, Mean Grey Value, and of course - limit to threshold.
can someone explain the differences between these measurments, especially regarding the above threshold areas relation to all selected area-
is Area - limited to threshold? is it normalised to the selected area?
Thanks
Tziona Ben-Gedalya,
Department of Cellular Biochemistry and Human Genetics
building 3 floor 0 room 33,
Hebrew university Hadassa Medical school,
Ein-Kerem, Jerusalem
[hidden email]
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