Hi Pablo,
> this C are the channel of my images?
Yes, the C dimension is the channels.
From your description, I do not understand your problem. Is it that you are
uncertain of the order of your channels, and since you cannot tell them
apart visually, you do not know which is which?
When you open your data using the Bio-Formats Importer plugin, then use
Show Info on your image, perhaps it will show more information about each
channel (such as emission wavelength) in the list? Depends on the file
format.
Regards,
Curtis
On Sun, Mar 10, 2013 at 6:58 PM, Pablo Munoz <
[hidden email]> wrote:
> Dear All
> I have a very simple question but cause me a lot of confusion , the
> question is the next.
> When I open my images with FIJI , I separate the channel but the program
> show me the images in greyscale and 8 bit and I take my images in 4 channel
> (sequential option in Leica microscopes) so when I open this images I only
> see in the upper part the letter C=1, C=2, C=3, C=4 so my doubt is
> regarding with this information, this C are the channel of my images?
> So then I can recognized my stain because I have same subcelular
> distribution with 2 markers so I can not distinguish it.
>
> Hope can someone give a guide
>
> Thank you for read and the patience
>
> Pablo
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