question about autobrightness settings?

[Jeremy Florman]

Hi,

My name is Micah, a PhD student and having an issue with quantifying GFP
image in image J. When I open this file (attached), the brightness and
contrast differ between what you see in preview and Image J. I believe
the preview appearance is correct because thats what I saw under the
microscope also. Has anyone run into this issue? Some how there is an
auto brightness setting that is changing the way my image appears in
ImageJ.

Help appreciated!!

Micah



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[ctrueden]

Hi Micah,

> the brightness and contrast differ between what you see in preview and
> Image J.

Short answer:
* Image > Adjust > Brightness/Contrast
* Set
* Min -> 0, Max -> 65535
* Now it will match what you see in Preview

Alternate approach:
* File > Import > Bio-Formats
* Choose your file
* Uncheck the "Autoscale" box
* Click OK
* Now it will match what you see in Preview

Explanation:
http://imagej.net/FAQ#The_image_colors_do_not_match_what_I_see_in_other_programs.21_ImageJ_is_wrong.21

Regards,
Curtis

On Wed, Feb 11, 2015 at 2:52 PM, Jeremy Florman <[hidden email]
> wrote:

> Hi,
>
> My name is Micah, a PhD student and having an issue with quantifying GFP
> image in image J. When I open this file (attached), the brightness and
> contrast differ between what you see in preview and Image J. I believe the
> preview appearance is correct because thats what I saw under the microscope
> also. Has anyone run into this issue? Some how there is an auto brightness
> setting that is changing the way my image appears in ImageJ.
>
> Help appreciated!!
>
> Micah
>
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html