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Hello everybody,
I am using the ImageJ to quantify protein on a 1-D gel.
However I am
experience some problems. When I select the "rectangular
selection tool"
to outline my marker, on the first line, it seems to be
okay. After
press 1, I move the box to outline the second line and press
2, and the
box come back to the line 1, so if I press 3, I will have
the same peak,
it means that I am analyzing twice the same band. I do not
know if my
explanation is clear, but if anyone have experienced this
situation and
have an idea how to solve this problem I will be glad.
Thanks for your attention.
Ivomar Oldoni
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