question with regard to image analysis

[Junghyo Jo]

Dear Image J experts,
I am wondering if ImageJ can analyze the following work?
I want to count double staining cells within a tissue boundary.

Although I am not familiar with ImageJ,
I imagine that to conduct this work,
ImageJ has to set the specific tissue boundary
on the sample image.
Then, it has to separately count two kinds of staining cells
which lies within the given tissue boundary.
Finally, it has to determine the double staining cells
based on the above information.
In the sample image, all nuclei of cells are stained with blue.
Is this procedure possible in ImageJ?

I appreciate your comment about this question.

Sincerely,
Junghyo

[Joel Sheffield]

Hi Junghyo,

In principle, the answer is yes.  As a practical matter, it will
depend very much on your images.  It would be very helpful if you
could post an example of your data for the list to see.

Joel

> Dear Image J experts,
> I am wondering if ImageJ can analyze the following work?
> I want to count double staining cells within a tissue boundary.
>
> Although I am not familiar with ImageJ,
> I imagine that to conduct this work,
> ImageJ has to set the specific tissue boundary
> on the sample image.
> Then, it has to separately count two kinds of staining cells
> which lies within the given tissue boundary.
> Finally, it has to determine the double staining cells
> based on the above information.
> In the sample image, all nuclei of cells are stained with blue.
> Is this procedure possible in ImageJ?
>
> I appreciate your comment about this question.
>
> Sincerely,
> Junghyo

--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
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http://astro.temple.edu/~jbs