re-organize an Image5D stack?

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re-organize an Image5D stack?

John Alexander-7
Does anyone know if it is possible to re-organize an Image5D stack?

I am using the LOCI plugin to open an image set (from a slidebook .sld
file).  It works great except that it always imports my z-series as a
time series.
This isn't normally a problem since they view the same way, except for
now I wish to use the image5D z-projection function and it wont work
because it thinks my z-series is a t-series.

So I am asking if anyone knows of a way to simply convert a 4-channel 21
slice "t-series" into the proper 4-channel 21 slice z-series.

I guess I could write a macro that would disassemble the Imag5D back
into stacks and then reassemble them properly ... but is there another way?

John
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Re: re-organize an Image5D stack?

Melissa Linkert
Hi John,

If your time-series files are being imported as Z-series, this is certainly
a bug in Bio-Formats.  If you would be willing to send one or more
problematic files, it would be very useful in fixing this issue.  Please let
me know if you need a place to store files - I can provide you with our FTP
server information off-list.

Regards,
-Melissa

On Mon, Mar 31, 2008 at 7:52 PM, John Alexander <[hidden email]>
wrote:

> Does anyone know if it is possible to re-organize an Image5D stack?
>
> I am using the LOCI plugin to open an image set (from a slidebook .sld
> file).  It works great except that it always imports my z-series as a
> time series.
> This isn't normally a problem since they view the same way, except for
> now I wish to use the image5D z-projection function and it wont work
> because it thinks my z-series is a t-series.
>
> So I am asking if anyone knows of a way to simply convert a 4-channel 21
> slice "t-series" into the proper 4-channel 21 slice z-series.
>
> I guess I could write a macro that would disassemble the Imag5D back
> into stacks and then reassemble them properly ... but is there another
> way?
>
> John
>