real-time plotting / live graphing of ROI

[caroliners]

Hello!

Is it possible to see a live plot of the ROI while doing a time-lapse series? If so, how?

Thank you!

p.s sorry if multiple-messages are sent. d.s

[Michael Schmid]

Hi Navid,

your question is not very clear.

Do you refer to a profile plot? Do you want to see it during  
acquisition of an image series, and if so, which plugins/software are  
you using?

If you have a normal ImageJ image window you can use the Dynamic  
Profiler plugin from the ImageJ web page to see a profile plot that  
gets updated each time the image changes.

Michael
________________________________________________________________

On 18 Jul 2011, at 14:21, caroliners wrote:

> Hello!
>
> Is it possible to see a live plot of the ROI while doing a time-lapse
> series? If so, how?
>
> Thank you!
>
> p.s sorry if multiple-messages are sent. d.s

[caroliners]

Hi Michael,

I apologize for not being specific enough.

I wish to follow my ROI live on a plot (with time on x-axis) when taking time-lapse photos, so I can follow changes in fluorescence live while it's recording.

Best wishes,
Navid

18 jul 2011 kl. 16:18 skrev Michael Schmid <[hidden email]>:

> Hi Navid,
>
> your question is not very clear.
>
> Do you refer to a profile plot? Do you want to see it during acquisition of an image series, and if so, which plugins/software are you using?
>
> If you have a normal ImageJ image window you can use the Dynamic Profiler plugin from the ImageJ web page to see a profile plot that gets updated each time the image changes.
>
> Michael
> ________________________________________________________________
>
> On 18 Jul 2011, at 14:21, caroliners wrote:
>
>> Hello!
>>
>> Is it possible to see a live plot of the ROI while doing a time-lapse
>> series? If so, how?
>>
>> Thank you!
>>
>> p.s sorry if multiple-messages are sent. d.s

... [show rest of quote]

[caroliners]

**

I'm using micromanager as sole plugin.

Navid

18 jul 2011 kl. 16:20 skrev "Michael Schmid-3 [via ImageJ]"<[hidden email]>:

Hi Navid,

your question is not very clear.

Do you refer to a profile plot? Do you want to see it during  
acquisition of an image series, and if so, which plugins/software are  
you using?

If you have a normal ImageJ image window you can use the Dynamic  
Profiler plugin from the ImageJ web page to see a profile plot that  
gets updated each time the image changes.

Michael
________________________________________________________________

On 18 Jul 2011, at 14:21, caroliners wrote:

> Hello!
>
> Is it possible to see a live plot of the ROI while doing a time-lapse
> series? If so, how?
>
> Thank you!
>
> p.s sorry if multiple-messages are sent. d.s

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... [show rest of quote]

[Michael Schmid]

Hi Navid,

does the Dynamic Profiler work on Micro-Manager while acquiring  
images? If yes, it would be fairly easy to create a similar plugin  
for your purpose; just taking the code of the Dynamic Profiler and  
replacing the code for obtaining a profile by ImageStatistics of the  
ROI. For plotting, one could take the time as x axis.

Michael
________________________________________________________________

On 18 Jul 2011, at 16:47, caroliners wrote:

> **
> I'm using micromanager as sole plugin.
>
>
> Navid

On 18 Jul 2011, at 16:44, Navid Soltani wrote:

> Hi Michael,
>
> I apologize for not being specific enough.
>
> I wish to follow my ROI live on a plot (with time on x-axis) when  
> taking time-lapse photos, so I can follow changes in fluorescence  
> live while it's recording.
>
> Best wishes,
> Navid
>
> 18 jul 2011 kl. 16:18 skrev Michael Schmid <[hidden email]>:
>
>> Hi Navid,
>>
>> your question is not very clear.
>>
>> Do you refer to a profile plot? Do you want to see it during  
>> acquisition of an image series, and if so, which plugins/software  
>> are you using?
>>
>> If you have a normal ImageJ image window you can use the Dynamic  
>> Profiler plugin from the ImageJ web page to see a profile plot  
>> that gets updated each time the image changes.
>>
>> Michael
>> ________________________________________________________________
>>
>> On 18 Jul 2011, at 14:21, caroliners wrote:
>>
>>> Hello!
>>>
>>> Is it possible to see a live plot of the ROI while doing a time-
>>> lapse
>>> series? If so, how?
>>>
>>> Thank you!
>>>
>>> p.s sorry if multiple-messages are sent. d.s

... [show rest of quote]

[caroliners]

Hello Michael!

Dynamic Profiler does work on micro-manager while acquiring images! How would one go about to create this plugin? I'm very new to coding, I looked through the code of Dynamic Profiler and the sourcecode of roi.class and imagestatistics.class, I'm not sure what to attempt to combine it with (or compile). Is this on the right way?

Thank you for your help.
Navid

On Mon, Jul 18, 2011 at 6:34 PM, Michael Schmid-3 [via ImageJ] <[hidden email]> wrote:

Hi Navid,

does the Dynamic Profiler work on Micro-Manager while acquiring  
images? If yes, it would be fairly easy to create a similar plugin  
for your purpose; just taking the code of the Dynamic Profiler and  
replacing the code for obtaining a profile by ImageStatistics of the  
ROI. For plotting, one could take the time as x axis.

Michael
________________________________________________________________

On 18 Jul 2011, at 16:47, caroliners wrote:

> **
> I'm using micromanager as sole plugin.
>
>
> Navid

On 18 Jul 2011, at 16:44, Navid Soltani wrote:

> Hi Michael,
>
> I apologize for not being specific enough.
>
> I wish to follow my ROI live on a plot (with time on x-axis) when  
> taking time-lapse photos, so I can follow changes in fluorescence  
> live while it's recording.
>
> Best wishes,
> Navid
>

> 18 jul 2011 kl. 16:18 skrev Michael Schmid <[hidden email]>:

>

>> Hi Navid,
>>
>> your question is not very clear.
>>
>> Do you refer to a profile plot? Do you want to see it during  
>> acquisition of an image series, and if so, which plugins/software  
>> are you using?
>>
>> If you have a normal ImageJ image window you can use the Dynamic  
>> Profiler plugin from the ImageJ web page to see a profile plot  
>> that gets updated each time the image changes.
>>
>> Michael
>> ________________________________________________________________
>>
>> On 18 Jul 2011, at 14:21, caroliners wrote:
>>
>>> Hello!
>>>
>>> Is it possible to see a live plot of the ROI while doing a time-
>>> lapse
>>> series? If so, how?
>>>
>>> Thank you!
>>>
>>> p.s sorry if multiple-messages are sent. d.s

... [show rest of quote]

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... [show rest of quote]