Hello!
Is it possible to see a live plot of the ROI while doing a time-lapse series? If so, how? Thank you! p.s sorry if multiple-messages are sent. d.s |
Hi Navid,
your question is not very clear. Do you refer to a profile plot? Do you want to see it during acquisition of an image series, and if so, which plugins/software are you using? If you have a normal ImageJ image window you can use the Dynamic Profiler plugin from the ImageJ web page to see a profile plot that gets updated each time the image changes. Michael ________________________________________________________________ On 18 Jul 2011, at 14:21, caroliners wrote: > Hello! > > Is it possible to see a live plot of the ROI while doing a time-lapse > series? If so, how? > > Thank you! > > p.s sorry if multiple-messages are sent. d.s |
Hi Michael,
I apologize for not being specific enough. I wish to follow my ROI live on a plot (with time on x-axis) when taking time-lapse photos, so I can follow changes in fluorescence live while it's recording. Best wishes, Navid 18 jul 2011 kl. 16:18 skrev Michael Schmid <[hidden email]>: > Hi Navid, > > your question is not very clear. > > Do you refer to a profile plot? Do you want to see it during acquisition of an image series, and if so, which plugins/software are you using? > > If you have a normal ImageJ image window you can use the Dynamic Profiler plugin from the ImageJ web page to see a profile plot that gets updated each time the image changes. > > Michael > ________________________________________________________________ > > On 18 Jul 2011, at 14:21, caroliners wrote: > >> Hello! >> >> Is it possible to see a live plot of the ROI while doing a time-lapse >> series? If so, how? >> >> Thank you! >> >> p.s sorry if multiple-messages are sent. d.s |
In reply to this post by Michael Schmid
** I'm using micromanager as sole plugin. Navid
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In reply to this post by caroliners
Hi Navid,
does the Dynamic Profiler work on Micro-Manager while acquiring images? If yes, it would be fairly easy to create a similar plugin for your purpose; just taking the code of the Dynamic Profiler and replacing the code for obtaining a profile by ImageStatistics of the ROI. For plotting, one could take the time as x axis. Michael ________________________________________________________________ On 18 Jul 2011, at 16:47, caroliners wrote: > ** > I'm using micromanager as sole plugin. > > > Navid On 18 Jul 2011, at 16:44, Navid Soltani wrote: > Hi Michael, > > I apologize for not being specific enough. > > I wish to follow my ROI live on a plot (with time on x-axis) when > taking time-lapse photos, so I can follow changes in fluorescence > live while it's recording. > > Best wishes, > Navid > > 18 jul 2011 kl. 16:18 skrev Michael Schmid <[hidden email]>: > >> Hi Navid, >> >> your question is not very clear. >> >> Do you refer to a profile plot? Do you want to see it during >> acquisition of an image series, and if so, which plugins/software >> are you using? >> >> If you have a normal ImageJ image window you can use the Dynamic >> Profiler plugin from the ImageJ web page to see a profile plot >> that gets updated each time the image changes. >> >> Michael >> ________________________________________________________________ >> >> On 18 Jul 2011, at 14:21, caroliners wrote: >> >>> Hello! >>> >>> Is it possible to see a live plot of the ROI while doing a time- >>> lapse >>> series? If so, how? >>> >>> Thank you! >>> >>> p.s sorry if multiple-messages are sent. d.s |
Hello Michael!
Dynamic Profiler does work on micro-manager while acquiring images! How would one go about to create this plugin? I'm very new to coding, I looked through the code of Dynamic Profiler and the sourcecode of roi.class and imagestatistics.class, I'm not sure what to attempt to combine it with (or compile). Is this on the right way? Thank you for your help. Navid On Mon, Jul 18, 2011 at 6:34 PM, Michael Schmid-3 [via ImageJ] <[hidden email]> wrote:
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