registration & rubbersheeting

[Cammer, Michael]

We are imaging muscle and tissue adjacent to muscle.  The problem is movement from two sources.  One is heartbeat.  Although we cannot synchronize the scanning, this is manageable.

The second is that the muscle twitches or undulates.  This means that in successive scans different parts of the field are more or less compressed than in preceding and succeeding timepoints.

There are enough fixed points in the tissue that if we could click manually on some number of points in each image, perhaps five or nine, and have these fit through the series to one reference image, our problem would be mostly solved.  Does anyone know of such software?

We're partial to ImageJ, but at this point we'd be interested to hear of any solutions.

Thank you!!!


_________________________________________
Michael Cammer, Optical Microscopy Specialist
http://ocs.med.nyu.edu/microscopy
Lab: [phone]  Cell: [phone]

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