One way to do this is to measure the centroid at each timepoint and translate the cell in X, Y over time so that the centroid is constant.
A few years ago we used this and also rotated the cells (sperm, based on the head of the sperm) so that we could measure the flagella movement.
===========================================================================
Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center
Cell: 914-309-3270 Lab: 212-263-3208
http://ocs.med.nyu.edu/microscopy &
http://www.med.nyu.edu/skirball-lab/dustinlab/-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Naama Hirsch
Sent: Wednesday, October 23, 2013 7:57 AM
To:
[hidden email]
Subject: registration
I need to measure processes coming out of a cell. However the cell is moving and I want to measure the process from the same point. Is there a way to do some kind of registration to the cell body?
thank you.
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