retinal color photographs and florescent anjiography images registration

[Murat Kucukevcilioglu]

i am an ophthalmologist and i am doing a study in retina patients who have a well known entity in the elderly known as age-related macular degeneration. main finding in this disease is accumulation in the back of the eye as small yellow spots named druse seen clinically. I am trying to compare the actual size of the druse from color photographs to corresponding fluorescence on the fluorescein angiography. i tried to register images but results were not so good. i m open to any advice....

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[Davíð Þór Bragason]

Hi Murat,

I am also working with fundus photo images in ImageJ, where I need to
register fundus photos taken at different wavelengths with the greatest
accuracy attainable -- sub-pixel if possible. I explored freely available
options, such the ones found in Fiji, but was not entirely happy with the
results. As you are working with different modalities (FA and color fundus
photos), that would probably be even more difficult.

There used to be a free project at Rensselaer called RIVERS which might
have been the best option for what you're doing. Unfortunately, this is no
longer available, but the tool is now a commercial product called "i2k
Retina" sold by DualAlign. I tried the free trial version and the results
were impressive. They do offer discounts for use in academic research which
I'm considering, as I would now like to register SLO images with fundus
photos. If someone knows about a free alternative, I would like to hear
about it.


On Tue, Jan 14, 2014 at 5:29 AM, Murat Kucukevcilioglu <
[hidden email]> wrote:

> i am an ophthalmologist and i am doing a study in retina patients who have
> a well known entity in the elderly known as age-related macular
> degeneration. main finding in this disease is accumulation in the back of
> the eye as small yellow spots named druse seen clinically. I am trying to
> compare the actual size of the druse from color photographs to
> corresponding fluorescence on the fluorescein angiography. i tried to
> register images but results were not so good. i m open to any advice....
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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[Murat Kucukevcilioglu]

Hi David
I want to ask something else. After registration can I quantify and compare the size of the each druse on color photo and fluorescein foto
Thankss

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Davíð Þór Bragason
Sent: Tuesday, January 14, 2014 12:20 PM
To: [hidden email]
Subject: Re: retinal color photographs and florescent anjiography images registration

Hi Murat,

I am also working with fundus photo images in ImageJ, where I need to register fundus photos taken at different wavelengths with the greatest accuracy attainable -- sub-pixel if possible. I explored freely available options, such the ones found in Fiji, but was not entirely happy with the results. As you are working with different modalities (FA and color fundus photos), that would probably be even more difficult.

There used to be a free project at Rensselaer called RIVERS which might have been the best option for what you're doing. Unfortunately, this is no longer available, but the tool is now a commercial product called "i2k Retina" sold by DualAlign. I tried the free trial version and the results were impressive. They do offer discounts for use in academic research which I'm considering, as I would now like to register SLO images with fundus photos. If someone knows about a free alternative, I would like to hear about it.


On Tue, Jan 14, 2014 at 5:29 AM, Murat Kucukevcilioglu < [hidden email]> wrote:

> i am an ophthalmologist and i am doing a study in retina patients who
> have a well known entity in the elderly known as age-related macular
> degeneration. main finding in this disease is accumulation in the back
> of the eye as small yellow spots named druse seen clinically. I am
> trying to compare the actual size of the druse from color photographs
> to corresponding fluorescence on the fluorescein angiography. i tried
> to register images but results were not so good. i m open to any advice....
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html


________________________________
Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.
________________________________

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[Murat Kucukevcilioglu]

Thanks for you help David

Do you know how I can quantify the druse size after aligning the color and FA images.

Best
Murat

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Davíð Þór Bragason
Sent: Tuesday, January 14, 2014 12:20 PM
To: [hidden email]
Subject: Re: retinal color photographs and florescent anjiography images registration

Hi Murat,

I am also working with fundus photo images in ImageJ, where I need to register fundus photos taken at different wavelengths with the greatest accuracy attainable -- sub-pixel if possible. I explored freely available options, such the ones found in Fiji, but was not entirely happy with the results. As you are working with different modalities (FA and color fundus photos), that would probably be even more difficult.

There used to be a free project at Rensselaer called RIVERS which might have been the best option for what you're doing. Unfortunately, this is no longer available, but the tool is now a commercial product called "i2k Retina" sold by DualAlign. I tried the free trial version and the results were impressive. They do offer discounts for use in academic research which I'm considering, as I would now like to register SLO images with fundus photos. If someone knows about a free alternative, I would like to hear about it.


On Tue, Jan 14, 2014 at 5:29 AM, Murat Kucukevcilioglu < [hidden email]> wrote:

> i am an ophthalmologist and i am doing a study in retina patients who
> have a well known entity in the elderly known as age-related macular
> degeneration. main finding in this disease is accumulation in the back
> of the eye as small yellow spots named druse seen clinically. I am
> trying to compare the actual size of the druse from color photographs
> to corresponding fluorescence on the fluorescein angiography. i tried
> to register images but results were not so good. i m open to any advice....
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html


________________________________
Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.
________________________________

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[Davíð Þór Bragason]

Hi Murat,

After the two images have been registered, you could draw the outlines of
the drusen on the FA and fundus photo images separately as ROIs using the
"freehand selections" tool. This would give you their respective areas in
square pixels, which would be directly comparable if all you need is their
relative sizes. If you need absolute sizes (e.g. square microns), then you
need to calibrate the images in some way, e.g. based on the size of the
optic disc or something like that.

Once you have the registered images, it can be helpful to make an image
stack out the them, which makes it easy to switch back and forth to get a
visual feel for the outline of the lesions on each image.

By the way, I noticed you're at U Iowa, in which case you could possibly
have access to in-house expertise in eye fundus image processing using
ImageJ from Michael Abramoff.

Good luck,
David



On Fri, Jan 17, 2014 at 6:11 PM, Kucukevcilioglu, Murat <
[hidden email]> wrote:

> Thanks for you help David
>
> Do you know how I can quantify the druse size after aligning the color and
> FA images.
>
> Best
> Murat
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> Davíð Þór Bragason
> Sent: Tuesday, January 14, 2014 12:20 PM
> To: [hidden email]
> Subject: Re: retinal color photographs and florescent anjiography images
> registration
>
> Hi Murat,
>
> I am also working with fundus photo images in ImageJ, where I need to
> register fundus photos taken at different wavelengths with the greatest
> accuracy attainable -- sub-pixel if possible. I explored freely available
> options, such the ones found in Fiji, but was not entirely happy with the
> results. As you are working with different modalities (FA and color fundus
> photos), that would probably be even more difficult.
>
> There used to be a free project at Rensselaer called RIVERS which might
> have been the best option for what you're doing. Unfortunately, this is no
> longer available, but the tool is now a commercial product called "i2k
> Retina" sold by DualAlign. I tried the free trial version and the results
> were impressive. They do offer discounts for use in academic research which
> I'm considering, as I would now like to register SLO images with fundus
> photos. If someone knows about a free alternative, I would like to hear
> about it.
>
>
> On Tue, Jan 14, 2014 at 5:29 AM, Murat Kucukevcilioglu <
> [hidden email]> wrote:
>
> > i am an ophthalmologist and i am doing a study in retina patients who
> > have a well known entity in the elderly known as age-related macular
> > degeneration. main finding in this disease is accumulation in the back
> > of the eye as small yellow spots named druse seen clinically. I am
> > trying to compare the actual size of the druse from color photographs
> > to corresponding fluorescence on the fluorescein angiography. i tried
> > to register images but results were not so good. i m open to any
> advice....
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
>
> ________________________________
> Notice: This UI Health Care e-mail (including attachments) is covered by
> the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is
> confidential and may be legally privileged.  If you are not the intended
> recipient, you are hereby notified that any retention, dissemination,
> distribution, or copying of this communication is strictly prohibited.
>  Please reply to the sender that you have received the message in error,
> then delete it.  Thank you.
> ________________________________
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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