roughness analysis

[nitinunv]

Dear,
I get the image of my film from AFM image (both in 2D and 3D manner). And I want to determine the roughness of the film (both z-axis and xy plane). Anyone have the idea how to figure it out?

I try a few plug-ins but the data is very confusing.

Thanks!
Bell

[Nathaniel Ryckman]

Here is a paper explaining roughness along with a link to the plugin they used/developed:

http://www.sciencedirect.com/science/article/pii/S0098300408001842
http://www.dentistry.bham.ac.uk/landinig/software/software.html

Here are a few more plugins:

http://fiji.sc/wiki/index.php/TopoJ
http://rsb.info.nih.gov/ij/plugins/roughness.html
http://www.gcsca.net/IJ/SurfCharJ.html

nitinunv wrote

Dear,
I get the image of my film from AFM image (both in 2D and 3D manner). And I want to determine the roughness of the film (both z-axis and xy plane). Anyone have the idea how to figure it out?

I try a few plug-ins but the data is very confusing.

Thanks!
Bell