Hi Karen,
(to the list: after some more discussion...)
In the ROI manager, if you select 'Show All', actually the image has
no ROI; 'show' does not mean 'select'. So, after 'Show All', no ROI
will be saved with the image.
You can easily see this by 'Show All' and doing some operation that
only affects the ROI such as 'Invert'. It will work on the whole
image, because there is no ROI.
Instead of 'Show All', you can use 'Combine', then you can save the
image with all the ROIs. There is also a 'Split' command in the
'More>>' menu of the ROI manager, it might be useful after opening
the saved image with the combined ROIs.
Michael
________________________________________________________________
On 26 Feb 2010, at 16:05, Karen Kempler wrote:
> I do have the latest version and I was saving as a tiff, but when I
> reopen the ROIs are not there.?? What am I doing wrong?
>
> Michael Schmid wrote:
>> Hi Karen,
>>
>> make sure you use the latest version and save as TIFF or ZIP.
>>
>> Michael
>> ________________________________________________________________
>>
>> On 26 Feb 2010, at 15:32, Karen Kempler wrote:
>>
>>> How do you save an image with the ROIs still in place? I have
>>> confocal images that I draw 3 ROIs on them and then I would like
>>> to save the image with those ROIs still on them, but I haven't
>>> been able to do that yet.
>>> Thank you
>>> Karen
>>
Locked
