segmentation

[Fabrice Senger]

Dear all,

I'do (at least I try) live imaging on transgenic animals. The cells
expressing a GFP tagged protein are compact, so it is quite difficult to
see what happens in terms of cell division and/or migration.
So im currently tryiing to stain the whole animal (Zebra), first results
are promising, and the stain I use should mainly stain the cell nucleus.

In terms of image ananlysis I would like to use the GFP channel, segment
GFP positiv cell masses and reuse this as a mask on the second channel
(stained nuclei), thus it should be possible to follow migration and
divisions.

Is there a 3D segemntation approach implemented in imageJ ?

Has someone experience in such an approach ?

Thanks for all comments and suggestions,

Fabrice.

[Thomas Boudier]

Hi Fabrice,

The Object Counter 3D with the "redirect to" option as in set
measurements/analyse particles does this perfectly.  Or if it is only
masking that you want use the imagecalculator and multiply (if your mask
is 0 and 1).

Best,

Thomas

> Dear all,
>
> I'do (at least I try) live imaging on transgenic animals. The cells
> expressing a GFP tagged protein are compact, so it is quite difficult
> to see what happens in terms of cell division and/or migration.
> So im currently tryiing to stain the whole animal (Zebra), first
> results are promising, and the stain I use should mainly stain the
> cell nucleus.
>
> In terms of image ananlysis I would like to use the GFP channel,
> segment GFP positiv cell masses and reuse this as a mask on the second
> channel (stained nuclei), thus it should be possible to follow
> migration and divisions.
>
> Is there a 3D segemntation approach implemented in imageJ ?
>
> Has someone experience in such an approach ?
>
> Thanks for all comments and suggestions,
>
> Fabrice.
>
>