setMinAndMax error

[Adrián Villalba]

 Dear all,

I am trying to apply the following to code to my tiff image.

run("Subtract Background...", "rolling=50 light");
run("Unsharp Mask...", "radius=2 mask=0.60");
run("Gamma...", "value=0.95");
run("Color Balance...");
setMinAndMax(-43, 211);


The problem is with the setMinAndMax function, when i apply this code i am
asked to put manually my min and max (and what i want is to set
automatically the color balance in the red channel between -43 and 211, but
i do not know why i should do it manually)

Does anyone know how can i range automatically my red channel to these
values? Thank you in advance!


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   - Adrián Villalba Felipe.
   https://es.linkedin.com/in/adrianvillalba

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[Herbie]

Good day,

the list of macro functions
<https://imagej.nih.gov/ij/developer/macro/functions.html>
tells us:

"*setMinAndMax(min, max, channels)*
Sets the display range of specified channels in an RGB image, where 4=red,
2=green, 1=blue, 6=red+green, etc. Note that the pixel data is altered since
RGB images, unlike composite color images, do not have a LUT for each
channel."

I don't know how you can assign a negative value because RGB is 0...255 in
each color channel.

HTH

Herbie



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[Adrián Villalba]

Dear Herbie, this is what i want to apply automatically (I extract the
negative value from the x axis in this screenshot). Do you know how can i
apply this in my macro?

Thank you,

2018-02-05 21:33 GMT+01:00 Herbie <[hidden email]>:

> Good day,
>
> the list of macro functions
> <https://imagej.nih.gov/ij/developer/macro/functions.html>
> tells us:
>
> "*setMinAndMax(min, max, channels)*
> Sets the display range of specified channels in an RGB image, where 4=red,
> 2=green, 1=blue, 6=red+green, etc. Note that the pixel data is altered
> since
> RGB images, unlike composite color images, do not have a LUT for each
> channel."
>
> I don't know how you can assign a negative value because RGB is 0...255 in
> each color channel.
>
> HTH
>
> Herbie
>
>
>
> --
> Sent from: http://imagej.1557.x6.nabble.com/
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--

   - Adrián Villalba Felipe.
   https://es.linkedin.com/in/adrianvillalba

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Herbie]

Does this

      setMinAndMax( -43, 211, 4 );

work for you?

Please note that you end up again with a range from 0 ... 255 per channel.

HTH

Herbie



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[Adrián Villalba]

No, it doesn't work.
The problem is not either the range, even with values between 0 and 255,
the setMinAndMax(x,y,4); command asks me for the color balance and i just
want the macro to do it automatically.

2018-02-05 22:03 GMT+01:00 Herbie <[hidden email]>:

> Does this
>
>       setMinAndMax( -43, 211, 4 );
>
> work for you?
>
> Please note that you end up again with a range from 0 ... 255 per channel.
>
> HTH
>
> Herbie
>
>
>
> --
> Sent from: http://imagej.1557.x6.nabble.com/
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--

   - Adrián Villalba Felipe.
   https://es.linkedin.com/in/adrianvillalba

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Herbie]

Sorry Dear,

but this *single* command

      setMinAndMax( -43, 211, 4 );

works for me as described.

No dialog, no asking ....

Clueless

Herbie

::::::::::::::::::::::::::::::::::::::::::::
Am 05.02.18 um 22:05 schrieb Adrián Villalba:

> No, it doesn't work.
> The problem is not either the range, even with values between 0 and 255,
> the setMinAndMax(x,y,4); command asks me for the color balance and i just
> want the macro to do it automatically.
>
> 2018-02-05 22:03 GMT+01:00 Herbie <[hidden email]>:
>
>> Does this
>>
>>        setMinAndMax( -43, 211, 4 );
>>
>> work for you?
>>
>> Please note that you end up again with a range from 0 ... 255 per
>> channel.
>>
>> HTH
>>
>> Herbie

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