simple question about tresholding

[Leoncio Vergara]

I am trying to do some intensity measurements using thresholding to exclude over and under saturated pixels on 16 bit images (0 and 65535 respectively). Whet I set the values to 1 and 65534, but they get reset to 0 and 65535. The min and max values within the mages are higher and lower than those limits respectively. Why is this happening? How can I can I do this correctly?

Thanks in advance for your help

Leo

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[Cammer, Michael-2]

I bet there is a better way to do this, but I think this would solve the problem via a macro:

run("32-bit");
changeValues(0,0,NaN);
changeValues(65535,65535,NaN);

Cheers-
Michael Cammer

-----Original Message-----
From: Leoncio Vergara <[hidden email]>
Sent: Thursday, June 6, 2019 6:17 PM
To: [hidden email]
Subject: simple question about tresholding

I am trying to do some intensity measurements using thresholding to exclude over and under saturated pixels on 16 bit images (0 and 65535 respectively). Whet I set the values to 1 and 65534, but they get reset to 0 and 65535. The min and max values within the mages are higher and lower than those limits respectively. Why is this happening? How can I can I do this correctly?

Thanks in advance for your help

Leo

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