Thanks a lot Michael!!
Rodrigo
________________________
From: Michael Schmid <
[hidden email]>
To:
[hidden email]
Sent: Thursday, 19 March 2015, 15:10
Subject: Re: size range in analyze particles?
Hi Rodrigo,
the maximum image size in ImageJ is 2^31-1 pixels = 2147483647 pixels (at least for ImageJ1)
So you can't have 30000000000.00 pixels.
If the area is in calibrated units (e.g. 30000000000 square micrometers, which is roughly 17 cm * 17 cm), the 'pixel' checkbox should be off, and the word 'pixel' should be removed from the macro command. Calibration of such an image in millimeters might be more handy.
Michael
________________________________________________________________
On Mar 19, 2015, at 16:13, Rodrigo Gonçalves wrote:
> Dear all,
>
> I need to measure particles with area bigger than 250 px2 and smaller than I want to avoid particles bigger than 30 000 000 000 px2.
>
> So I analyze particles in the image:
> run("Analyze Particles...", "size=250.00-30000000000.00 pixel show=Outlines display clear summarize add");
>
> But in the Results window list I obtain one particle (#3) bigger than the set size(44493121880.74 px2), Also, when I select the “big” particle in the ROI and I press “measure”, I even get a different (but still bigger than desired) area: 53494241962.58 px2
>
>
>
> What am I doing wrong?
>
> Thanks, Rodrigo
> image:
> sample_th.tif
>
> ________________________
> Rodrigo J. Gonçalves
> | |
> | | | | | | | |
> | sample_th.tifShared with Dropbox |
> | |
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> | |
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>
>
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