software binning

[Richard Cole]

Dear Group

Is there a way to software bin an image?  I have a large format CCD camera
that I would like to software bin to increase brightness.

Thanks

RWC




 
Richard Cole
Research Scientist IV
Director: Advanced Light Microscopy & Image Analysis Core
Wadsworth Center
P.O. Box 509 Albany N.Y. 12201-0509
* 518-474-7048
** 518-474-4430  
* [hidden email]
Website www.wadsworth.org/cores/alm/index.htm
 
 




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[Harry Parker]

Hello RWC.
I'm not sure what you mean by "software binning". How is it different than simply adding multiple short exposure images together?
(Adding images is very simple in ImageJ.)
 
--  
Harry Parker  
Senior Systems Engineer  
Digital Imaging Systems, Inc.

----- Original Message ----
From: Richard Cole <[hidden email]>
To: [hidden email]
Sent: Wednesday, December 26, 2007 7:16:30 AM
Subject: software binning


Dear Group

Is there a way to software bin an image?  I have a large format CCD
 camera
that I would like to software bin to increase brightness.

Thanks

RWC
 
Richard Cole
Research Scientist IV
Director: Advanced Light Microscopy & Image Analysis Core
Wadsworth Center
P.O. Box 509 Albany N.Y. 12201-0509
* 518-474-7048
** 518-474-4430  
* [hidden email]
Website www.wadsworth.org/cores/alm/index.htm
 





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[Michael Schmid]

Hi Richard,

you need two steps:
(1) multiply the image by a factor of your choice, e.g. 4.
(2) use the Averaging Reducer plugin (on the ImageJ web site)
to reduce the image size by, e.g. a factor of 2 in each direction.

With the multiplication factor of 4, this will be equivalent to
2x2 binning.

To automate it, do it manually and let the Macro Recorder (Plugins>
Macros>Record) create a macro for you.

Michael
______________________________________________________________________
On Wed, 26 Dec 2007 07:16:30 -0500 Richard Cole <[hidden email]>
wrote:

>Dear Group
>
>Is there a way to software bin an image?  I have a large format CCD camera
>that I would like to software bin to increase brightness.
>
>Thanks
>
>RWC
>
>Richard Cole
>Research Scientist IV
>Director: Advanced Light Microscopy & Image Analysis Core
>Wadsworth Center
>P.O. Box 509 Albany N.Y. 12201-0509
>* 518-474-7048
>** 518-474-4430  
>* [hidden email]
>Website www.wadsworth.org/cores/alm/index.htm
>
>IMPORTANT NOTICE: This e-mail and any attachments may contain
>confidential or sensitive information which is, or may be, legally
>privileged or otherwise protected by law from further disclosure.  It
>is intended only for the addressee.  If you received this in error or
>from someone who was not authorized to send it to you, please do not
>distribute, copy or use it or any attachments.  Please notify the
>sender immediately by reply e-mail and delete this from your
>system. Thank you for your cooperation.
>

[Michael Schmid]

Hi Joel,

as the name says, the Averaging Reducer calculates an average over
n by n pixels.

Image>Adjust Size: Without "interpolate" it takes the pixel nearest
to the position corresponding to the new pixel center.
With "interpolate", it uses linear interpolation between the nearest
pixels of the original image.

Thus, when downscaling by a factor of 5, the Averaging Reducer will
always take the average of 5x5 = 25 pixels as a new pixel.
Image>Adjust Size will never use more than 4 pixels (those closest
to the position that the output pixel should correspond to).

Adjust Size is better in preserving edges, but it can lead to
aliasing (e.g. missing thin lines) and it does not significantly
reduce the noise.

In theory, the "ideal" downscaling algorithm would be applying a
bandwith-limiting filter first (some sort of sin(x)/x multiplied
by a window function to avoid infinite ringing) and then downsampling
with proper interpolation. But I don't think that anyone has
implemented this so far.
(There is also a downscaling algorithm in GaussianBlur for large
blur radii: This one is bandwidth-limited, but it attenuates high
spatial frequencies more than one would like for downscaling an
image without blurring it).

Michael
__________________________________________________________________

On Sun, 30 Dec 2007 13:46:21 -0500 "Joel Sheffield" <[hidden email]>
wrote:

>Michael,
>
>I have been enjoying your comments for some time.  This latest
>suggestion brings up a question.  What is the difference between the
>averaging reducer plugin and the scale function that it built into
>ImageJ?  The difference as shown on the web site for the plugin is
>very dramatic.
>
>Thanks, and Happy New Year.
>
>Joel
>
>Date sent:       Sun, 30 Dec 2007 11:24:16 +0100
>Send reply to:   ImageJ Interest Group <[hidden email]>
>From:           Michael Schmid <[hidden email]>
>Subject:         Re: software binning
>To:             [hidden email]
>
>> Hi Richard,
>>
>> you need two steps:
>> (1) multiply the image by a factor of your choice, e.g. 4.
>> (2) use the Averaging Reducer plugin (on the ImageJ web site)
>> to reduce the image size by, e.g. a factor of 2 in each direction.
>>
>> With the multiplication factor of 4, this will be equivalent to
>> 2x2 binning.
>>
>> To automate it, do it manually and let the Macro Recorder (Plugins>
>> Macros>Record) create a macro for you.
>>
>> Michael
>> ______________________________________________________________________
>> On Wed, 26 Dec 2007 07:16:30 -0500 Richard Cole <[hidden email]>
>> wrote:
>> >Dear Group
>> >
>> >Is there a way to software bin an image?  I have a large format CCD camera
>> >that I would like to software bin to increase brightness.
>> >
>> >Thanks
>> >
>> >RWC
>> >
>> >Richard Cole
>> >Research Scientist IV
>> >Director: Advanced Light Microscopy & Image Analysis Core
>> >Wadsworth Center
>> >P.O. Box 509 Albany N.Y. 12201-0509
>> >* 518-474-7048
>> >** 518-474-4430  
>> >* [hidden email]
>> >Website www.wadsworth.org/cores/alm/index.htm
>> >
>> >IMPORTANT NOTICE: This e-mail and any attachments may contain
>> >confidential or sensitive information which is, or may be, legally
>> >privileged or otherwise protected by law from further disclosure.  It
>> >is intended only for the addressee.  If you received this in error or
>> >from someone who was not authorized to send it to you, please do not
>> >distribute, copy or use it or any attachments.  Please notify the
>> >sender immediately by reply e-mail and delete this from your
>> >system. Thank you for your cooperation.
>> >
>
>Joel B. Sheffield, Ph.D
>Department of Biology
>Temple University
>Philadelphia, PA 19122
>Voice: 215 204 8839
>e-mail: [hidden email]
>URL:  http://astro.temple.edu/~jbs
>