I'd like to average the frames in a stack but all I can find are "walking"
averagers where they will average say frames1-4 then 2-5 etc.. what I'd like to do is take the average of frames 1-4 then 5-8 and so on, so that we can take data that is say at a frame fate of 4 fps and make it look like it was taken at 1 fps (to reduce the noise in our images and because we record things at different frame rates). So essentially if we were doing say 4 frame averaging then a 100 frame stack would turn in to a 25 frame stack where each slice was the average of 4 consecutive frames. I could write a plugin to do this, but if one exists, or there is a macro I could use can some one point me in the right direction ? thanks.. -Jeff -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
Dear Jeff,
Sounds like you might be looking for Grouped Z-Projection; Image > Stacks > Tools > Grouped Z-Projection Best wishes Kees Dr Ir K.R. Straatman Senior Experimental Officer Advanced Imaging Facility Centre for Core Biotechnology Services University of Leicester http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif -----Original Message----- From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Jeff Spector Sent: 10 December 2014 02:55 To: [hidden email] Subject: stack averaging I'd like to average the frames in a stack but all I can find are "walking" averagers where they will average say frames1-4 then 2-5 etc.. what I'd like to do is take the average of frames 1-4 then 5-8 and so on, so that we can take data that is say at a frame fate of 4 fps and make it look like it was taken at 1 fps (to reduce the noise in our images and because we record things at different frame rates). So essentially if we were doing say 4 frame averaging then a 100 frame stack would turn in to a 25 frame stack where each slice was the average of 4 consecutive frames. I could write a plugin to do this, but if one exists, or there is a macro I could use can some one point me in the right direction ? thanks.. -Jeff -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
In reply to this post by Jeff Spector-2
Dear Jeff,
How about using the "Walking Average" plugin you mention, then only use every 4th image. Regards David Strachan -----Original Message----- From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Jeff Spector Sent: 10 December 2014 02:55 To: [hidden email] Subject: stack averaging I'd like to average the frames in a stack but all I can find are "walking" averagers where they will average say frames1-4 then 2-5 etc.. what I'd like to do is take the average of frames 1-4 then 5-8 and so on, so that we can take data that is say at a frame fate of 4 fps and make it look like it was taken at 1 fps (to reduce the noise in our images and because we record things at different frame rates). So essentially if we were doing say 4 frame averaging then a 100 frame stack would turn in to a 25 frame stack where each slice was the average of 4 consecutive frames. I could write a plugin to do this, but if one exists, or there is a macro I could use can some one point me in the right direction ? thanks.. -Jeff -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
Thanks everyone for the advice. It looks like either a grouped Z projection
or using transform->bin, and then using the z-dimension will both work for me. -Jeff On Wed, Dec 10, 2014 at 5:41 AM, David Strachan < [hidden email]> wrote: > Dear Jeff, > > How about using the "Walking Average" plugin you mention, then only use > every 4th image. > > > Regards > David Strachan > > > -----Original Message----- > From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of > Jeff Spector > Sent: 10 December 2014 02:55 > To: [hidden email] > Subject: stack averaging > > I'd like to average the frames in a stack but all I can find are "walking" > averagers where they will average say frames1-4 then 2-5 etc.. what I'd > like to do is take the average of frames 1-4 then 5-8 and so on, so that we > can take data that is say at a frame fate of 4 fps and make it look like it > was taken at 1 fps (to reduce the noise in our images and because we record > things at different frame rates). So essentially if we were doing say 4 > frame averaging then a 100 frame stack would turn in to a 25 frame stack > where each slice was the average of 4 consecutive frames. > I could write a plugin to do this, but if one exists, or there is a macro > I could use can some one point me in the right direction ? > thanks.. > -Jeff > > -- > ImageJ mailing list: http://imagej.nih.gov/ij/list.html > > -- > ImageJ mailing list: http://imagej.nih.gov/ij/list.html > -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
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