stacking zvi Axio files

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stacking zvi Axio files

Giagtzoglou, Nikolaos
Dear all,
I have saved zvi files from Axio software. It is amazing that there is a plugin available for opening this format in Image J
Usually, we use the commercial software CZ focus to stack all images together in one.
I tried to do that by applying every different method that exists for stacking in ImageJ, but the image was not nearly as clear as the one that we ususally got.
I was wondering whether I have any parameters set up wrong or there is a plugin that I might not be aware of
Thank you for your attention
 
 

Nikos

 
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Re: stacking zvi Axio files

ctrueden
Hi Nikos,

If your zvi files are numbered sequentially, you can check the "Group files
with similar names" checkbox in the Bio-Formats Importer plugin options to
read them in all at once as a stack. Otherwise, after reading them in
individually, you can use the Image > Stacks > Images to Stacks command to
convert all the standalone image planes into a single image stack. Depending
on the organization of your data other plugins might be useful too such as
the Concatenator, Stack Combiner or other stack-related plugin from <
http://rsb.info.nih.gov/ij/plugins/#stacks>.

-Curtis

On Jan 24, 2008 10:10 AM, Giagtzoglou, Nikolaos <[hidden email]>
wrote:

> Dear all,
> I have saved zvi files from Axio software. It is amazing that there is a
> plugin available for opening this format in Image J
> Usually, we use the commercial software CZ focus to stack all images
> together in one.
> I tried to do that by applying every different method that exists for
> stacking in ImageJ, but the image was not nearly as clear as the one that we
> ususally got.
> I was wondering whether I have any parameters set up wrong or there is a
> plugin that I might not be aware of
> Thank you for your attention
>
>
>
> Nikos
>
>
>